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目的观察急性淋巴细胞白血病儿童在化疗结束后T淋巴细胞亚群的变化,了解T淋巴细胞重建过程。方法采用流式细胞仪分析76名ALL治疗后停药不同阶段的儿童外周血T淋巴细胞亚群。结果CD3+HLA-DR+细胞、CD4+CD45RO-CD45RA-细胞百分率在停药后0~12月组高于对照组(P<0·05),CD3+CD4-CD8+、CD8+CD28-细胞百分率在停药后0~12月、12~24月24~48月组均高于对照组,CD3+CD8-CD4+、CD4+CD45RO-CD45RA+细胞的百分率、CD4/CD8值、CD45RA/CD45RO值在停药各个阶段与对照组相比明显降低(P<0·05)。结论化疗结束后,活化的T细胞可能在停药早期免疫系统的监视作用中起积极的作用,患儿的T淋巴细胞恢复需要较长的时间。CD4+CD45RO-CD45RA+、CD3+CD4+CD8-淋巴细胞的恢复最慢。
Objective To observe the changes of T lymphocyte subsets in children with acute lymphoblastic leukemia after chemotherapy and to understand the process of T lymphocyte remodeling. Methods Flow Cytometry was used to analyze T lymphocyte subsets in peripheral blood of 76 children who were treated with ALL after discontinuation of drug therapy. Results The percentages of CD3 + HLA-DR + cells and CD4 + CD45RO-CD45RA- cells in the 0-12 month group after stopping treatment were significantly higher than those in the control group (P0.05). The percentage of CD3 + CD4-CD8 + and CD8 + The percentages of CD3 + CD8-CD4 + and CD4 + CD45RO-CD45RA + cells, CD4 / CD8 and CD45RA / CD45RO after stopping treatment from 0 to 12 months and from 12 to 24 months from 24 to 48 months after stopping treatment were higher than those of the control group All stages were significantly lower than those in the control group (P <0.05). Conclusion After chemotherapy, activated T cells may play an active role in monitoring the early immune system, and T lymphocytes in children need longer time to recover. CD4 + CD45RO-CD45RA +, CD3 + CD4 + CD8- lymphocytes recovered the slowest.