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据笔者试验,稻瘟病菌(生长在酵母、淀粉、洋菜培养基上)置冰箱(7℃)内可存活13个月以上。这种方法保存下的菌株,因预先完成了斜面培养,可直接挑入大麦粒培养基中繁殖孢子,较之病稻节干燥保藏法获得孢子快而方便。 方法 取24×200毫米试管,内装20毫升培养基(琼脂20克,可溶性淀粉10克,酵母2克,水1000毫升),灭菌后搁成斜面,把从江西大余县地江乡采集的水稻品种龙10病穗中分离的两个单孢(编号23-1、23-2)各移入1个试管,菌丝长满斜面后,于1986
According to the author test, Magnaporthe grisea (growth in yeast, starch, agar medium) home refrigerator (7 ℃) can survive more than 13 months. Strain preserved by this method, because of pre-completed bevel culture, can be directly picked into the spore of barley grain medium, compared with the Wistaria dry preservation method to obtain spores fast and convenient. Methods A 24 × 200 mm tube was filled with 20 ml of medium (20 g of agar, 10 g of soluble starch, 2 g of yeast, and 1000 ml of water). After sterilizing, put it into a bevel and collect it from Dijiang Township, Dayu County, Jiangxi Province Two single spores (No. 23-1,23-2) isolated from the panicle of rice variety Long 10 were transferred to a test tube. After the mycelium was full of bevel, in 1986