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目的研究γ干扰素(IFN-γ)对神经干细胞的增殖和自我更新能力的影响;Wnt7a蛋白在IFN-γ促神经干细胞增殖和增强自我更新能力的机制。方法从胎鼠端脑获取原代神经干细胞进行原代培养,分别添加一定浓度IFN-γ溶液和PBS溶液,分为IFN-γ处理组和PBS对照组,计算神经球体面积,细胞免疫荧光检测神经干细胞Brd U增值能力,蛋白免疫印迹检测神经干细胞IFN-γ信号通路蛋白p STAT1和Wnt7a信号通路蛋白。结果形态学显示IFN-γ增加神经球面积和Brd U+细胞数目,与对照组相比差异有统计学意义(P<0.01);蛋白免疫印迹结果显示IFN-γ促进了p STAT1和Wnt7a蛋白增加,与对照组相比差异有统计学意义(P<0.01)。结论 IFN-γ增强了神经干细胞的自我更新能力和增殖能力,并且激活了Wnt7a信号通路。
Objective To investigate the effect of IFN-γ on the proliferation and self-renewal of neural stem cells and the mechanism of Wnt7a protein on the proliferation and self-renewal of neural stem cells induced by IFN-γ. Methods Primary neural stem cells (NSCs) were harvested from fetal rat telencephalon and cultured in primary culture. IFN-γ and PBS solutions were respectively added into IFN-γ group and PBS control group to calculate the area of neurospheres and the immunofluorescence Stem cells Brd U value-added ability, Western blot detection of neural stem cells IFN-γ signaling pathway protein p STAT1 and Wnt7a signaling pathway protein. RESULTS: Morphology showed that IFN-γ increased the area of neurospheres and the number of Brd U + cells compared with the control group (P <0.01). Immunoblotting results showed that IFN-γ promoted the increase of protein of p STAT1 and Wnt7a, Compared with the control group, the difference was statistically significant (P <0.01). Conclusion IFN-γ enhances the self-renewal ability and proliferation ability of neural stem cells, and activates the Wnt7a signaling pathway.