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为了从紫花苜蓿伴生菌中筛选生物防治菌种,采用对峙法和滤液培养法对紫花苜蓿伴生菌与菌核病菌的拮抗关系进行了研究,并采用茎秆接种法探讨了紫花苜蓿伴生菌对菌核病菌的控制作用。从紫花苜蓿植株中分离获得的22种真菌中,射丝孢、无孢菌丝体和丝葚霉对菌核病菌具有明显的拮抗作用;接种第5天,在含射丝孢、无孢菌丝体和丝葚霉3种真菌菌液的培养基上,三叶草核盘菌的菌落直径分别为4.8、26.8和28.9 mm,与对照相比,分别降低了75.2、53.2和51.1 mm。虽然射丝孢和无孢菌丝体都不能阻止菌核病菌侵入寄主组织,但对菌核病菌入侵后病斑的扩展表现出不同程度的抑制作用,其效果因品种而异,2种菌种对品种FGC901和Algonguin病斑扩展均表现出较好的抑制作用。
In order to screen out the biological control strains from the alfalfa companion bacteria, the antagonistic relationship between the alfalfa associated bacteria and the sclerotial bacteria was studied by the confrontation method and the filtrate culture method. The effects of alfalfa companion bacteria Control of nuclear bacteria. Among the 22 fungi isolated from alfalfa plants, R. solani, S. aurantiacus and D. mulberry showed obvious antagonism against S. sclerotiorum. On the 5th day after inoculation, The diameters of colonies of S. sclerotiorum were 4.8, 26.8 and 28.9 mm, respectively, which were decreased by 75.2, 53.2 and 51.1 mm respectively compared with the control. Although neither Sporella nor sporeless mycelium could prevent the invasion of Sclerotinia sclerotiorum into the host tissues, it showed different degrees of inhibition on the spread of Sclerotinia sclerotiorum after invaded by S. sclerotiorum. The effects of two species On the varieties of FGC901 and Algonguin lesion expansion showed better inhibition.