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目的了解野生动物市场果蝠携带SARS冠状病毒(SARS-CoV)和SARS样冠状病毒(SARS-like-CoV)情况。方法2004年9月至2005年11月,在广州及广州野生动物市场收集来自广州及周边地区的分属两个亚目9个种的蝙蝠共905只,有犬蝠(Cynopterussphinx)、棕果蝠(Rousettusleschenaulti)、普通长翼蝠(Miniopterusschreibersi)、普氏蹄蝠(Hipposiderospratti)、中华菊头蝠(Rhinolophussinicus)、小黄蝠(Scotophiluskuhlii)、大耳双色蹄蝠(HipposiderosPomona)、中菊头蝠(Rhinolophusaffinis)和小菊头蝠(Rhinolophuspusillus)。收集的蝙蝠分别取得咽拭813份、血清524份、肺组织853份及直肠粪便853份标本共计3043份,用新型冠状病毒(N-蛋白)测定试剂盒(酶联免疫法)、SARS-CoVRNA检测试剂盒、荧光PCR及基因芯片检测并通过RT-PCR和细胞分离培养检测SARS-CoV和SARS-like-CoV。结果收集的9个种905只蝙蝠共3043份标本未检测、分离出SARS-CoV和SARS-like-CoV。结论调查的广州及周边地区的9种蝙蝠均未检测到SARS-CoV和SARS-like-CoV。
Objective To understand the situation of fruit flocks carrying SARS-CoV and SARS-like coV in wildlife markets. METHODS: From September 2004 to November 2005, 905 bats belonging to 9 species of two sub-families from Guangzhou and the surrounding areas were collected from Guangzhou and Guangzhou wild animal markets. Cynopterussphinx, Rousettusleschenaulti, Miniopterusschreibersi, Hipposiderospratti, Rhinolophussinicus, Scotophilus kuhlii, Hipposideros pomona, Rhinolophus affinis ) And Rhinolophus pusillus. The collected bats were obtained from 813 swabs, 524 serums, 853 lungs and 853 rectal samples for a total of 3043 samples. The new coronavirus (N-protein) assay kit (enzyme-linked immunosorbent assay), SARS-CoVRNA Detection kit, fluorescence PCR and gene chip detection and detection of SARS-CoV and SARS-like-CoV by RT-PCR and cell isolation and culture. Results A total of 3043 specimens of 905 bats collected from 9 batches were not detected, SARS-CoV and SARS-like-CoV were isolated. Conclusions SARS-CoV and SARS-like-CoV were not detected in all 9 bats in Guangzhou and surrounding areas.