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为了更方便高效地进行线粒体DNA高变区测序结果的分析统计 ,利用Internet上的共享资源 ,建立了两种普通PC机上可以实现的分析途径 :一种是利用bioedit软件对测序文件进行读取、校正 ,然后转换为文本文件导入MicrosoftExcel与Anderson标准序列进行比对并进行相应突变位置、变异碱基筛选、单倍型频率和基因多样性等的统计。另一种是利用Chromas、Seqvert er、Clustalx和Genedoc软件对线粒体DNA与标准序列进行比对 ,确定突变的位置和数目。两种途径所用的软件界面简单明了 ,结果直观明确 ,对线粒体DNA的分析统计有很强的实用意义。
In order to more conveniently and efficiently analyze the sequencing results of mitochondrial DNA hypervariable region, we can establish the analysis approaches that can be implemented on two kinds of common PC using the shared resources on the Internet: one is to read the sequencing files by bioedit software, After calibration, and then converted into a text file into MicrosoftExcel and Anderson standard sequences were aligned and the corresponding mutation location, mutation base screening, haplotype frequency and gene diversity and other statistics. The other is the use of Chromas, Seqvert er, Clustalx and Genedoc software for mitochondrial DNA and standard sequences were aligned to determine the location and number of mutations. The software interfaces used by the two approaches are simple and clear, the results are intuitive and clear, and the analysis and statistics of mitochondrial DNA have strong practical significance.