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目的观察胃得安片对大鼠应激性胃溃疡炎性因子和胃酸分泌抑制作用,探讨其治疗胃溃疡的作用机制。方法大鼠随机分为正常对照组和造模组,造模组分为模型对照组(等容量氯化钠注射液),阳性对照组(盐酸雷尼替丁)和胃得安片低、中、高剂量组,每组10只。各组大鼠每天灌胃给药1次,连续给药2周。采用空腹冷水游泳法制备大鼠胃溃疡模型;采用ELISA法测定血清IL-6、IL-2、TNF-α、生长抑素、胃泌素、再生蛋白Ⅰ(RegⅠ)、增殖细胞核抗原(PCNA)含量。结果与模型对照组比较,各给药组RegⅠ和PCNA的含量均明显降低(P<0.05,P<0.01);各给药组大鼠血清胃泌素、TNF-α、IL-6和IL-2含量显著降低(P<0.05,P<0.01),生长抑素含量显著升高(P<0.05),其中胃得安片高、中剂量组差异最为显著,呈现一定剂量依赖性。结论胃得安片能够减少胃酸分泌,降低血清炎性因子水平,具有一定的抑酸、抗炎作用。
Objective To observe the effect of Weidean An on inhibitory factor of gastric ulcer and gastric acid secretion in gastric ulcer in rats and explore its mechanism of action in treating gastric ulcer. Methods The rats were randomly divided into normal control group and model group. The model group was divided into model control group (equal capacity sodium chloride injection), positive control group (ranitidine hydrochloride) , High dose group, 10 rats in each group. Rats in each group were given gavage once daily for 2 weeks. The rats model of gastric ulcer was established by fasting cold water swimming. The serum levels of IL-6, IL-2, TNF-α, somatostatin, gastrin, RegⅠ and PCNA were determined by ELISA. content. Results Compared with the model control group, the contents of RegⅠ and PCNA in each administration group were significantly decreased (P <0.05, P <0.01). Serum gastrin, TNF-α, IL-6 and IL- (P <0.05, P <0.01), and the content of somatostatin increased significantly (P <0.05). The difference between the two groups was significant, with the dose-dependent manner. Conclusion Weidean An Tablet can reduce gastric acid secretion, reduce serum levels of inflammatory cytokines, with certain inhibition of acid and anti-inflammatory effects.