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目的通过超声提取工艺及HPLC法比较胶质和粉质重楼总皂苷及其皂苷单体的含量差异。方法以总皂苷得率为指标,正交试验优化重楼总皂苷的超声提取工艺后,按照优选的工艺对6批次重楼总皂苷、皂苷单体进行HPLC含量测定。色谱条件:Agilent C18柱(4.6mm×250mm,5μm),流动相:乙腈-水,梯度洗脱:0~40min,30%~65%乙腈,流速:0.8ml·min-1,检测波长:203nm。结果在超声提取条件下,最佳工艺为:乙醇浓度60%,料液比1∶50,提取时间为30min,胶质重楼总皂苷含量高于粉质重楼;6种皂苷单体HPLC检测条件下标准曲线线性良好(r>0.9998),粉质重楼6种皂苷单体含量与胶质重楼存在显著差异。结论建立的方法准确、可靠、稳定,为重楼药材的质量控制提供科学依据,并为胶质和粉质重楼的合理使用提供了数据支持。
OBJECTIVE To compare the content of total saponin and its saponin monomer in the gum and silty reticulum by ultrasonic extraction and HPLC. Methods The total saponin yield was used as an index to optimize the ultrasonic extraction process of the total saponins of Rhizoma Paridis by orthogonal test. The HPLC method was used to determine the total saponins and saponins of six batches. Chromatographic conditions: Agilent C18 column (4.6 mm × 250 mm, 5 μm), mobile phase: acetonitrile-water with gradient elution: 0-40 min, 30% -65% acetonitrile, flow rate: 0.8 ml · min -1, detection wavelength: 203 nm . Results Under the conditions of ultrasonic extraction, the optimum conditions were: ethanol concentration 60%, solid-liquid ratio 1:50, extraction time 30 min, total saponin content Under the conditions of the standard curve of a good linear (r> 0.9998), Saponin content of six kinds of saponin monomer content and glial floor were significantly different. Conclusion The established method is accurate, reliable and stable, and provides a scientific basis for the quality control of Rhizoma Paridis. It also provides data support for the rational use of glial and silty re-establishment.