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目的 探讨丹参注射液联合5-氟尿嘧啶(5-Fu)对小鼠Lewis肺癌细胞凋亡的作用。方法 将60只Lewis肺癌荷瘤C57BL/6小鼠随机分为模型组,丹参高、低剂量组(丹高组、丹低组),5-Fu组,丹参高、低剂量联合5-Fu组(丹低+5-Fu组、丹高+5-Fu组),每组10只。各组于接种第2日起每天腹腔注射给药0.2ml/只,5-Fu组连续给药5天,联合组先给5-Fu及丹参注射液混合液5天,后继续给予丹参注射液5天。其余各组均连续给药10天。末次给药24h后摘眼球取血再脱颈椎处死,观察血常规、瘤体重量、肺癌细胞凋亡指数、主要脏器转移灶数目及病理改变。结果 丹参低剂量组和丹参高剂量组瘤体重量较模型组降低(P<0.05),5-Fu组、丹参低剂量+5-Fu、高剂量+5-Fu组较模型组瘤体重量明显减轻(P<0.01)。丹参高剂量组、5-Fu组、丹参低剂量+5-Fu组及丹参高剂量+5-Fu组瘤体细胞凋亡指数与模型组比均增高(P<0.05)。结论 丹参注射液能够增强5-Fu对肿瘤的抑制,有增强小鼠Lewis肺癌细胞凋亡作用的趋势。
Objective To explore the effect of Salvia miltiorrhiza injection combined with 5-fluorouracil on the apoptosis of Lewis lung carcinoma cells in mice. Methods Sixty Lewis lung cancer bearing C57BL / 6 mice were randomly divided into model group, Salvia miltiorrhiza high and low dose group (Danao group and Danxia group), 5-Fu group, high and low dose Salvia miltiorrhiza combined with 5-Fu group (Dan low + 5-Fu group, Dan Gao + 5-Fu group), 10 in each group. Each group on the second day after inoculation daily administration of 0.2ml / only, 5-Fu group for 5 consecutive days of administration, the combined group of 5-Fu and Salvia injection mixture for 5 days, then continue to give Salvia injection 5 days. The remaining groups were administered for 10 consecutive days. After the last administration, the eyeballs were removed and the cervical vertebrae were sacrificed. The blood routine, the weight of the tumor, the apoptotic index of lung cancer, the number of metastatic organs and the pathological changes were observed. Results Compared with the model group, the tumor weight of Salvia miltiorrhiza low dose group and Salvia miltiorrhiza high dose group decreased (P <0.05), and the tumor weight of 5-Fu group, Salvia miltiorrhiza low dose + 5-Fu group and high dose + 5- Reduce (P <0.01). Salvia miltiorrhiza high dose group, 5-Fu group, Salvia miltiorrhiza low dose + 5-Fu group and Salvia miltiorrhiza high dose + 5-Fu group apoptosis index and model group were higher (P <0.05). Conclusion Salvia miltiorrhiza injection can enhance 5-Fu on tumor inhibition, and enhance the apoptosis of Lewis lung cancer cells in mice.