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A simple,eco-friendly.sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosidcs.The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β-hydroxyethyl)rutosides fluorescence enhancement,increasing considerably the sensitivity of detection.All the variables affecting the fluorescence intensity were studied and optimized.The flow rate was 5 mL/min with detection at 450 nm(after excitation at 346 nm).A linear correlation between drug amount and peak area was established for 0-(β-hydroxyethyl)rulosides in the range of 0.01-200 μg/mL with a detection limit of0.001 μg/mL(s/n = 3).Validation processes were performed by recovering studies with satisfactory results.The new methodology can be employed for the routine analysis of 0-(P-hydroxyethyl)rutosides in bulks as well as in commercial formulations.
A simple, eco-friendly. Positive and economic flow injection spectrofluorimetric method was developed for the determination of O- (β-hydroxyethyl) rutosidc. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL / min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for 0- (β-hydroxyethyl) rulosides in the range of 0.01-200 μg / mL with a detection limit of 0.001 μg / mL (s / n = 3) .Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of 0- (P-hydroxyethyl) rutosides in bulks as well as in commercial formulations.