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目的 了解ＡｐｏＥ基因多态性在中老年人群中的分布。方法 采用多聚酶链反应限制性片段长度多态性技术（ＰＣＲ－ＲＦＬＰ），检测广州市荔湾区５１２ 名年龄５５ 岁及以上的健康志愿者的ＡｐｏＥ基因型，特异性扩增ＡｐｏＥ基因第四外显子区含编码１１２ 位至１５８ 位氨基酸的基因序列，产物片段２４４ ｂｐ，用限制性内切酶ＨｈａＩ酶解ＰＣＲ产物，应用聚丙烯酰胺凝胶电泳进行酶解产物的基因分型。结果 广州市荔湾区健康中老年人群中，ＡｐｏＥ等位基因频率分别为ε２∶９．２％ ，ε３∶８４．２％ ，ε４∶６．６％ ，ε２ 频率随年龄增加有上升趋势，其中６５～７４ 岁年龄组与５５～６４ 岁年龄组比较，差异有显著性意义（Ｐ＜ ０．０５），ε４ 频率则随年龄增加而明显下降（Ｐ＜ ０．０５）。ＡｐｏＥ等位基因频率分布与男女性别无关。结论 广州市荔湾区健康中老年人群中的ＡｐｏＥ等位基因频率的分布与国内其他多数健康人群大体相同，与国外同类研究相比则有差异。
Objective To understand the distribution of ApoE gene polymorphism in the middle-aged and elderly population. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the ApoE genotype of 512 healthy volunteers aged 55 years and over in Guangzhou Liwan District, The sub-region contained a gene sequence encoding amino acids 112 to 158, a fragment of 244 bp. The PCR product was digested with restriction enzyme HhaI and polyacrylamide gel electrophoresis was used to genotype the digested product. Results The frequencies of ApoE alleles were ε2: 9.2%, ε3:84.2%, ε4:6.6%, ε2, and ε2, respectively, in healthy middle-aged and elderly population in Liwan District, Guangzhou City. Among them, 65 There was a significant difference between the age group of ~ 74 and the age group of 55 ~ 64 (P <0.05), while the frequency of ε4 decreased with age (P <0.05). ApoE allele frequency distribution has nothing to do with gender. Conclusion The frequencies of ApoE alleles in healthy middle-aged and elderly population in Liwan District of Guangzhou are similar to those of most other healthy people in China, but they are different from those in other foreign countries.