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目的:了解远红外线对造血细胞增殖和定向分化的影响方法:应用体外小鼠骨髓细胞培养技术,观察远红外线对粒-单祖细胞(CFU-GM)、红系祖细胞(CFU-E)和成纤维细胞(CFU-F)增殖和分化的影响,并且利用免疫荧光纳米粒子(IFNB)检测靶细胞表面分化抗原(CD),进一步评估远红外线对造血干/祖细胞的影响结果:远红外线在37℃条件下照射对CFU-E、CFU-F和CFU-GM生成有促进作用,小鼠骨髓细胞表面标记有变化,靶细胞经2min照射后,增殖最旺盛2 min组与其它实验组(1 min,5 min,10min)数据经统计学处理,具有差异性(P均<0.05)。结论:远红外线对小鼠骨髓造血干/祖细胞的增殖具有正调节作用并可诱导细胞表面标志改变
Objective: To investigate the effects of far infrared radiation on proliferation and differentiation of hematopoietic cells.Methods: In vitro mouse bone marrow cell culture was used to observe the effects of far infrared radiation on CFU-GM, erythroid progenitor cells (CFU-E) and Fibroblasts (CFU-F) proliferation and differentiation, and the use of immunofluorescent nanoparticles (IFNB) detection of target cell surface differentiation antigen (CD), to further assess the effect of far infrared on hematopoietic stem / progenitor cells results: far infrared in At 37 ℃, CFU-E, CFU-F and CFU-GM production were promoted by irradiation, and the surface markers of mouse bone marrow cells were changed. After 2min irradiation, the target cells proliferated most vigorously for 2min and the other experimental groups (1 min, 5 min, 10 min) were statistically different (all P <0.05). Conclusion: Far-infrared radiation can positively regulate the proliferation of mouse bone marrow hematopoietic stem / progenitor cells and induce changes of cell surface markers