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以模式植物拟南芥的β–微管蛋白为参考依据,通过电子克隆的方法,从西洋梨基因组中克隆了9个β–微管蛋白基因,并根据苹果和梨基因组间的保守性及共线性原理,实现了这些基因的染色体定位。序列分析显示,这组基因均含有3个外显子和两个内含子。起源于全基因组复制事件而形成的成对染色体上的基因不仅在外显子和内含子长度上具有较高的保守性,而且编码的氨基酸残基也有更高的序列相似性。对9个β–微管蛋白基因在矮生梨与普通梨杂种群体中的荧光定量表达分析表明,有7个基因在茎尖组织中表达,其中定位于chr16上的转录本号为PCP044487.1的基因在矮生型和普通型梨茎尖中的转录水平出现极显著差异,推测该基因可能与梨矮生性状形成的分子调控有重要关系。
Based on the β-tubulin of model plant Arabidopsis thaliana, nine β-tubulin genes were cloned from the pear genome by the method of electronic cloning. Based on the conservation of the apple and pear genomes and the total Linear principle, to achieve the chromosomal location of these genes. Sequence analysis showed that this group of genes contain three exons and two introns. Genes originating from genome-wide duplication events on paired chromosomes are not only highly conserved in exon and intron lengths, but also have higher sequence similarity in the encoded amino acid residues. The quantitative analysis of the expression of nine β-tubulin genes in the dwarf pear and common pear hybrid population showed that seven genes were expressed in the shoot tip tissue, and the transcript number located on chr16 was PCP044487.1 The results showed that there was a significant difference in the transcriptional level between the stems of dwarf and common pears. It is speculated that this gene may play an important role in the molecular regulation of the formation of pear dwarf traits.