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背景:白细胞流变性对微循环血流灌注有明显影响,但缺氧对微循环中白细胞流变性的影响目前研究极少。目的:研究急、慢性缺氧对大鼠肠系膜微循环白细胞流变学行为的影响。设计:随机对照的实验研究。地点、材料和干预:本实验在解放军第三军医大学高原军事医学系全军高原医学重点实验室进行。成年Wistar大鼠,校动物中心提供,按随机抽签法分为对照组、急性缺氧组和慢性缺氧组,低压舱模拟海拔5000m连续缺氧2,30d,复制动物急性和慢性缺氧模型。主要观察指标:正常大鼠、急性缺氧大鼠、慢性缺氧大鼠的白细胞滚动速度、沿壁滚动数、黏附数和白细胞-内皮细胞接触时间(leukocyte-endotheliumcontacttime,TLECT)及动脉血气的比较结果。结果:与对照组比较,急、慢性缺氧组动物表现为动脉血氧分压PaO2和血氧饱和度SaO2显著下降(t=3.01～8.30,P<0.05)。对照组白细胞滚动速度(101.41±26.96)μm/s,滚动数(4.27±2.69)个/min,黏附数(1.45±1.21)个/100μm,TLECT(0.91±0.73)s/min;急性缺氧动物白细胞滚动速度(85.44±17.33)μm/s,滚动数(60.00±31.62)个/min,黏附数(26±8.22)个/100μm,TLECT(16.30±4.84)s/min;慢性缺氧白细胞滚动速度(72.40±24.08)μm/s,滚动数(14.43±3.99)个/min,黏附数(3.57±1.27)个/100μm,TLECT(2
BACKGROUND: Leukocyte rheology has a significant effect on microcirculation perfusion, but the effect of hypoxia on leukocyte rheology in microcirculation is poorly studied. Objective: To study the effects of acute and chronic hypoxia on the rheological behavior of mesenteric microcirculation leukocytes in rats. Design: Randomized controlled experimental study. Location, Materials and Interventions: This experiment was conducted in the PLA Military Medical Laboratory of Plateau Military Medicine, Third Military Medical University. The adult Wistar rats were provided by School Animal Center and were randomly divided into control group, acute hypoxia group and chronic hypoxia group randomly. The hypoxia model was used to simulate the acute and chronic hypoxia model of animals continuously at hypoxia for 2 days and 5000 days. MAIN OUTCOME MEASURES: Comparison of white blood cell rolling velocity, rolling along the wall, adhesion number and leukocyte-endothelium contact time (TLECT) and arterial blood gas in normal rats, acute hypoxic rats and chronic hypoxic rats result. Results: Compared with the control group, the arterial partial pressure of oxygen (PaO2) and the oxygen saturation (SaO2) of acute and chronic hypoxia groups decreased significantly (t = 3.01-8.30, P <0.05). The number of rolling (4.27 ± 2.69) / min, the number of adhesion (1.45 ± 1.21) /100μm, andTLECT (0.91 ± 0.73) s / min were significantly higher in the control group than those in the control group (101.41 ± 26.96μm / s) The number of rolling (60.00 ± 31.62) / min, the number of adhesion (26 ± 8.22) /100μm, TLECT (16.30 ± 4.84) s / min, the rate of white blood cells rolling at a speed of 85.44 ± 17.33 μm / s, (72.40 ± 24.08) μm / s, rolling number (14.43 ± 3.99) / min, adhesion number (3.57 ± 1.27)