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目的:观察运动和丝瓜络干预对糖尿病肾病大鼠水液代谢的影响,并探讨其水通道蛋白调节机制。方法:40只雄性SD大鼠,随机分为4组:正常对照组、模型组、丝瓜络组、丝瓜络运动组,以链脲佐菌素诱导建立糖尿病模型,给予丝瓜络灌胃或丝瓜络联合运动。取血液样本测定各组血糖、尿素氮,ELISA法测血液AVP水平,取尿液样本测定尿蛋白排泄率,ELISA法测尿液AQP-2水平,采用Western blot法测定肾髓质AQP-2蛋白表达。结果:糖尿病大鼠血糖、血清、尿素氮、AVP水平及尿蛋白排泄率增高,尿液AQP-2浓度显著增高,肾脏髓质AQP-2蛋白表达上调,丝瓜络能显著降低模型大鼠血糖、血清、尿素氮和AVP水平,显著降低尿液AQP-2浓度和肾髓质AQP-2蛋白表达;结合运动后血清尿素氮及尿液AQP-2水平较丝瓜络组显著降低,但肾髓质AQP-2表达变化无统计学意义。结论:运动结合丝瓜络干预可改善糖尿病大鼠水液代谢紊乱,其机制可能与调节AQP-2表达有关。
Objective: To observe the effects of exercise and loofah intervention on water-fluid metabolism in rats with diabetic nephropathy and to explore its mechanism of aquaporin regulation. Methods: Forty male SD rats were randomly divided into 4 groups: normal control group, model group, loofah group and loofah group. Diabetic rats were induced by streptozotocin, Joint movement. Blood samples were taken to measure the blood glucose and urea nitrogen. The level of AVP in blood was measured by ELISA. The urinary protein excretion rate was measured by urine samples. The level of AQP-2 in urine was measured by ELISA. AQP-2 protein expression. Results: The levels of AQP-2 and AQP-2 in urine of diabetic rats were increased, the levels of AQP-2 and AQP-2 were increased, while the levels of blood glucose, serum urea nitrogen, AVP and urinary protein excretion were increased, Serum urea nitrogen and AVP levels significantly reduced urine AQP-2 concentration and renal medulla AQP-2 protein expression; combined exercise serum urea nitrogen and urine AQP-2 levels were significantly lower than loofah group, but the medullary AQP-2 expression was not statistically significant. Conclusion: The combination of exercise and loofah can improve the disturbance of water-fluid metabolism in diabetic rats, which may be related to the regulation of AQP-2 expression.