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目的:研究 PTTG 和 c-myc 基因表达在原发性肝细胞癌 (HCC)发生发展中的作用.方法:应用原位杂交(DNA-RNA)技术与免疫组化 SP 法分别检测61例原发性肝细胞癌及癌旁肝组织中 PTTG mRNA 和PTTG 蛋白及 c-myc mRNA 和 c-myc 蛋白的表达.结果:在原发性肝细胞癌(HCC)中,PTTG mRNA 和 PTTG蛋白阳性细胞呈弥漫性、小巢状或散在分布,在胞质内呈全浆型、膜下型表达.PTTG mRNA 和 PTTG 蛋白在 HCC 中表达率分别为72.1%(44/61)和78.7%(48/61),在癌旁肝组织中分别为93.4%(57/61)和91.8%(56/61),在 HCC中表达明显低于癌旁肝组织(P<0.005,P<0.05).相关性检验显示癌及癌旁 PTTG 基因表达与 c-myc 基因表达呈正相关(P<0.005).结论:PTTG 基因过度表达参与了肝细胞癌发生发展,过度表达的 FTTG 可能通过激活癌基因 c-myc 来参与肝细胞恶性转化和肝细胞癌的发生发展过程.
Objective: To investigate the role of PTTG and c-myc gene in the development of primary hepatocellular carcinoma (HCC) .Methods: In situ hybridization (DNA-RNA) and immunohistochemistry (SP) PTTG, PTTG, c-myc and c-myc in hepatocellular carcinoma and para-cancerous liver tissue.Results: In primary hepatocellular carcinoma (HCC), PTTG mRNA and PTTG positive cells were Diffuse, small nests or scattered, and expressed in the cytoplasm in plasma and subtype.The expressions of PTTG mRNA and PTTG protein in HCC were 72.1% (44/61) and 78.7% (48/61) respectively ) And 93.4% (57/61) and 91.8% (56/61) respectively in the adjacent non-cancerous liver tissues, which were significantly lower in HCC than in para-cancerous liver tissues (P <0.005, P <0.05) (P <0.005) .Conclusion: PTTG gene overexpression is involved in the development of hepatocellular carcinoma, and overexpression of FTTG may be involved in the activation of oncogene c-myc Hepatic malignant transformation and development of hepatocellular carcinoma.