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目的为探讨化学诱导舌癌和食管癌的作用及可能机制,利用化学致癌剂4硝基喹啉1氧化物(4NQO)建立C57BL/6小鼠的舌癌和食管癌模型。方法 100μg/ml、50μg/ml、10μg/ml的4NQO通过饮水法作用于C57BL/6小鼠,实验期间通过肉眼和组织学观察病变过程。结果随着摄入4NQO浓度的增加,小鼠在同一时期的发病率也随之增加,病变加重,同时随着服用时间的延长,病情逐渐加剧。在实验过程中,可以观察到舌和食管的病变经历了单纯性上皮增生—异常增生—原位癌—浸润性癌这样一个经典的鳞状上皮细胞癌的病变过程。当服用100μg/ml4NQO16周,到第28周时可观察到浸润性癌的病理症状。结论采用4NQO饮水法成功建立了C57BL/6小鼠舌癌和食管癌动物模型,为研究口腔鳞状上皮细胞癌和食管鳞状上皮细胞癌提供了有价值的动物模型。
Objective To investigate the role of chemosensitive tongue and esophageal carcinomas and their possible mechanisms, tongue cancer and esophageal cancer models of C57BL / 6 mice were established by using 4NQO as chemical carcinogen. Methods C57BL / 6 mice were treated with 4NQO at 100μg / ml, 50μg / ml and 10μg / ml by drinking water method. The pathological changes were observed by naked eye and histology during the experiment. Results As the concentration of 4NQO increased, the incidence of mice in the same period also increased, the lesions increased, and with the extension of taking time, the condition gradually aggravated. During the experiment, it was observed that the lesions of the tongue and esophagus underwent the pathological process of a classic squamous cell carcinoma, such as simple epithelial hyperplasia - dysplasia - carcinoma in situ - invasive carcinoma. When taking 100 μg / ml 4NQO for 16 weeks, pathological symptoms of invasive carcinomas were observed by the 28th week. Conclusion The animal model of tongue cancer and esophageal cancer in C57BL / 6 mice was successfully established by 4NQO drinking water method, which provided a valuable animal model for the study of oral squamous cell carcinoma and esophageal squamous cell carcinoma.