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实验选用霸王无菌苗的茎尖、子叶、下胚轴和胚根作为材料,研究霸王不同外植体的离体培养技术。结果表明,霸王茎尖是诱导丛生芽的良好外植体,而子叶、下胚轴和胚根是诱导愈伤组织的良好外植体;霸王茎尖的最适增殖培养基是:MS+6-BA 5.0 mg.L-1+NAA 1.0 mg.L-1;最适生根培养基是:MS+IAA 1.5 mg.L-1;最适愈伤组织诱导培养基是:MS+6-BA 1.0 mg.L-1或MS+6-BA 1.0 mg.L-1+NAA 0.5 mg.L-1。沙质基质为霸王组培苗过渡的最佳基质。
In the experiment, shoot tip, cotyledon, hypocotyl and radicle of aseptic seedlings of Bawang were selected as materials to study the in vitro culture technology of different explants. The results showed that bulge shoot tip was a good explant for inducing shoots, while cotyledon, hypocotyl and radicle were good explants for inducing callus. The optimum proliferation medium of bulbul shoot tip was MS + 6 -BA 5.0 mg.L-1 + NAA 1.0 mg.L-1. The optimal medium for rooting was MS + IAA 1.5 mg.L-1. The optimal callus induction medium was MS + 6-BA 1.0 mg.L-1 or MS + 6-BA 1.0 mg.L-1 + NAA 0.5 mg.L-1. Sand matrix for the King of tissue culture seedling transition best matrix.