论文部分内容阅读
建立快速、简捷、灵敏的LC-ESI/MS法测定人血浆中枸橼酸喷托维林的浓度,并用于含此种成分的复方愈酚维林胶囊的药代动力学与生物等效性的研究。血浆样品经液-液萃取后以甲醇与含0.4%冰乙酸和4mmol·L-1乙酸铵的水(43∶57,v/v)为流动相,采用ESI源以SIM方式对血浆样品中枸橼酸喷托维林进行定量分析。研究结果表明,枸橼酸喷托维林血浆浓度测定方法的线性范围为1～160ng·mL-1,日内、日间精密度(RSD)均小于12.5%,方法回收率为92%～110%,提取回收率大于80%。本法操作简便、快速、灵敏度高、专属性强,满足生物样本的分析要求,可用于枸橼酸喷托维林的生物等效性与药代动力学研究。
To establish a rapid, simple and sensitive LC-ESI / MS method for the determination of pentobarbitone citrate in human plasma and to study its pharmacokinetics and bioequivalence Research. Plasma samples were extracted by liquid-liquid extraction with methanol and 0.4% glacial acetic acid and 4mmol·L-1 ammonium acetate in water (43:57, v / v) as mobile phase. Citrate Pentoxyverine for quantitative analysis. The results showed that the linear range of the method for the determination of pentotaveline citrate was 1 ~ 160 ng · mL-1, the intra-day and inter-day precision was less than 12.5%, and the recovery was 92% -110% , Extraction recovery rate of more than 80%. The method is simple, rapid, sensitive and specific. It meets the biological sample analysis requirements and can be used to study the bioequivalence and pharmacokinetics of pentobarbital citrate.