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目的:探讨三氧化二砷与氨氯地平单独用药及联合用药对肝癌Hep G2细胞增殖和凋亡的作用。方法:用不同浓度的三氧化二砷(4.0、2.0、1.0μmol/L)和氨氯地平(27×103、18×103、12×103 mg/m L)处理体外培养的人肝癌Hep G-2细胞,观察细胞形态的变化,采用CCK-8法检测两种药物单独及联合应用对Hep G-2细胞生长增殖的影响,并通过流式细胞术观察其对细胞凋亡及细胞周期的影响。结果:三氧化二砷和氨氯地平均可以浓度依赖性方式显著增加Hep G2细胞的生长抑制率及其凋亡率,以联合用药的作用较单独用药更显著(P<0.05)。与三氧化二砷和氨氯地平单独用药相比,联合用药可显著阻滞Hep G2细胞于G2期及S期。结论:三氧化二砷和氨氯地平均可显著抑制人肝癌Hep G2细胞的生长和增殖,并促进其凋亡,且二者联合应用时具有协同作用。
Objective: To investigate the effects of arsenic trioxide and amlodipine alone and in combination on the proliferation and apoptosis of Hep G2 cells. Methods: Human hepatocellular carcinoma Hep G-2 cells were treated with various concentrations of arsenic trioxide (4.0,2.0,1.0μmol / L) and amlodipine (27 × 103,18 × 103,12 × 103 mg / mL) The changes of cell morphology were observed. The effects of two drugs alone and in combination on the growth and proliferation of Hep G-2 cells were detected by CCK-8 assay. The effects of two drugs on apoptosis and cell cycle were observed by flow cytometry. Results: Both arsenic trioxide and amlodipine significantly increased the growth inhibitory rate and apoptosis rate of Hep G2 cells in a concentration-dependent manner. The effect of combination therapy was more significant than that of drug alone (P <0.05). Compared with arsenic trioxide and amlodipine alone, combination therapy significantly blocked Hep G2 cells in G2 and S phases. CONCLUSION: Both arsenic trioxide and amlodipine can significantly inhibit the growth and proliferation of Hep G2 cells and promote the apoptosis of Hep G2 cells, both of which have a synergistic effect.