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目的:为研究起搏机需辨认,计数和分离大鼠窦房结起搏(P)细胞.方法:根据最早起搏点和细胞电位记录确定大鼠窦房结中央区位置,取窦房结连续切片.HE和Masson染色,以光镜,图像仪及电镜辨认、分析窦房结内各类细胞形态特点和各类细胞数量.结果:从细胞大小,外形,染色浓淡,核/胞浆比,核面积和核灰度,肌原纤维分化程度等指标,可区分各类细胞,并算出各类细胞的数量比.结论:确立了各类细胞的辨别标准,中央区P细胞总数1200～2000个,占细胞总数的13.1%.过渡细胞占44%.纤维细胞占17.1%.
Objective: To study the pacing machine need to identify, count and separate rat sinoatrial node pacemaker (P) cells.Methods: According to the earliest pacemaker and cell potential record to determine the central site of the rat sinoatrial node location, take the sinoatrial node Serial sections were stained with HE and Masson, and the morphological characteristics and cell numbers of various types of cells were analyzed by light microscope, imager and electron microscope.Results: From the cell size, appearance, , Nuclear area and nuclear gray degree, myofibrillar differentiation and other indicators, can distinguish between various types of cells, and calculate the number of various types of cells than.Conclusion: established the identification criteria of various types of cells, the central area of the total number of P cells 1200 ~ 2000 Accounting for 13.1% of the total number of cells, transitional cells accounting for 44%, fibroblasts accounting for 17.1%.