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目的 探讨碱性磷酸酶(ALP)及同工酶与肾脏疾病的关系。方法 1997 -03 ~2003 -05黑龙江省医院用自动生化分析仪测定43例肾脏病患者ALP浓度,聚丙烯酰胺凝胶圆盘电泳及扫描定量测定ALP同工酶浓度。结果 按肾脏病理改变分为肾小球损伤组、肾小管损伤组和重度弥漫病变组。尿液总ALP肾小球损伤组和肾小管损伤组与对照组比较差异有极显著性(P<0 .001),组织非特异型ALP同工酶与正常对照组比较差异有极显著性(P<0. 001);尿液小肠型ALP同工酶肾小管损伤组与正常对照组和肾小球损伤组比较差异有极显著性(P<0. 001)。严重病变组尿液总ALP及同工酶与正常对照组比较差异无显著性(P>0. 05 )。结论 尿液ALP同工酶可以作为肾脏不同部位损伤的指标。
Objective To investigate the relationship between alkaline phosphatase (ALP) and isozymes and renal diseases. Methods 1997 -03 ~ 2003 -05 Hospital of Heilongjiang Province with automatic biochemical analyzer determination of 43 patients with renal disease ALP concentration, polyacrylamide gel electrophoresis and disk scanning electrophoresis ALP isoenzyme levels. The results were divided into renal glomerulus pathological changes of glomerular injury group, renal tubular injury group and severe diffuse lesion group. Urinary total ALP glomerular injury and tubular injury compared with the control group were significantly different (P <0 .001), tissue nonspecific ALP isozymes compared with the normal control group was significantly different (P < P <0.001). Urinary small intestine type ALP isoenzymes in renal tubular injury group compared with normal control group and glomerular injury group were significantly different (P <0.001). There was no significant difference in total ALP and isoenzyme in urine between severe disease group and normal control group (P> 0.05). Conclusion Urinary ALP isozymes can be used as an indicator of injury in different parts of the kidney.