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目的了解牛磺酸对缺铁性贫血大鼠铁生物利用度的影响,并探讨其可能的机制。方法将大鼠分为3组:正常对照组(NG)和经贫血造模之后的缺铁性贫血组[缺铁性贫血组分为牛磺酸组(TG)和实验对照组(EG)]。3组给予补铁饲养25d,同时TG每天灌胃牛磺酸。根据所摄入铁量和血红蛋白(Hb)值比较TG和EG对铁的生物利用度,并算出TG的相对效价。同时测定铁营养代谢相关的生化指标血清铁浓度,不饱和铁结合力(UIBC),总铁结合力(TIBC)和转铁蛋白饱和度(TS)。用蛋白质印迹法观察肝组织hepcidin的表达。结果TG对铁的生物利用度高于EG,其相对效价(RBV)为126%。TG的其他生化指标接近NG,且明显优于EG。TG肝组织hepcidin的表达高于NG,低于EG。结论牛磺酸可能是通过抑制肝组织hepcidin表达,提高大鼠对铁的生物利用度,从而更有效地改善缺铁性贫血。
Objective To understand the effect of taurine on iron bioavailability in iron deficiency anemia rats and to explore its possible mechanism. Methods The rats were divided into 3 groups: normal control group (NG) and iron deficiency anemia group after anemia model [iron deficiency anemia group: taurine group (TG) and experimental control group (EG)] . Three groups were given iron supplementation for 25 days, meanwhile, TG was fed into taurine everyday. The bioavailability of TG and EG to iron was compared on the basis of iron intake and hemoglobin (Hb) values and the relative potency of TG was calculated. Serum iron concentration, UIBC, TIBC and TS of biochemical indicators of iron nutrition metabolism were also determined. Western blotting was used to observe hepcidin expression in liver tissue. Results The bioavailability of TG to iron was higher than that of EG, with a relative potency (RBV) of 126%. TG other biochemical indicators close to NG, and significantly better than EG. TG hepatic hepcidin expression higher than NG, lower than EG. Conclusion Taurine may improve iron deficiency bioavailability in rats by inhibiting the expression of hepcidin in liver tissues and thus improve iron deficiency anemia more effectively.