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目的 探讨内蒙籍汉族儿童过敏性紫癜 (AP)关节、胃肠道、肾脏受累与HLA DQA1等位基因的相关性。方法 采用聚合酶链反应 序列特异性引物 (PCR SSP)技术 ,在内蒙籍汉族无血缘关系及其他风湿性疾病史或家族史的人群中 ,收集儿童AP累及关节 45例、胃肠道 39例和肾脏 32例分别与 90名健康儿童HLA DQA1等位基因作型别分析。结果 AP累及关节、胃肠道和肾脏DQA1 0 30 1等位基因频率分别为 2 8 5 %、2 4 9%和 2 5 % ,均明显高于对照组 10 6 % ,差异有非常显著性 (χ2 分别为 12 0 0 8、7 6 39和 6 882 ,P值分别为 0 0 0 1、0 0 0 6和 0 0 0 9,均 <0 0 1,RR分别为3 83、3 0 9和 3 11,EF分别为 0 36、0 2 95和 0 2 97) ;而DQA1 0 30 2等位基因频率分别为 5 7%、3 9%和 6 5 % ,明显低于对照组 19% ,差异有非常显著性 (χ2 分别为 8 35 2、10 6 33和 5 5 5 7,P值分别为 0 0 0 4、0 0 0 2和 0 0 18,前二者P <0 0 1,后者P <0 0 5 ,RR分别为 0 2 4、0 15 9和 0 2 72 ,PF分别为 1 0 9、1 80和 0 92 )。结论 HLA DQA1 0 30 1等位基因可能是内蒙籍汉族儿童AP累及关节、胃肠道和肾脏发病单体型中一个遗传易感基因 ,而DQA1 0 30 2等位基因可能为其遗传保护基因
Objective To investigate the association between HLA DQA1 alleles and the involvement of joints, gastrointestinal tract and kidneys in children with Henoch-schonlein purpura (AI) in Inner Mongolia. Methods Polymerase chain reaction-sequence-specific primers (PCR SSP) were used to detect the association of AP with 45 cases of joint, 39 cases of gastrointestinal tract and 40 cases of gastrointestinal tract in the Han nationality with no history of blood relationship and other rheumatic diseases or family history in Inner Mongolia. 32 cases of kidney and 90 healthy children HLA DQA1 allele for type analysis. Results The frequencies of allele DQA1 0 30 1 in the joints, gastrointestinal tract and kidneys were 28.5%, 24.9% and 25% respectively, which were significantly higher than those in the control group χ2 were 12 0 0 8, 7 6 39 and 6 882 respectively, with P values of 0 0 0 1 0 0 0 6 and 0 0 0 9 respectively (all <0 0 1, RR 3 83 and 0 9 0, respectively) 3 11, and EF respectively 0 36,0 2 95 and 0 2 97). However, the frequency of DQA 1 0 30 2 allele was 57%, 39% and 65% respectively, which was significantly lower than that of the control group (19% (Χ2 = 835 2,106 33 and 5 5 5 7 respectively, P values were 0 0 0 4 0 0 0 2 and 0 0 18 respectively, P <0 01 for the latter two P <0 0 5, RR 0 2 4, 0 15 9 and 0 2 72 respectively, with PFs of 109, 80 and 0 92, respectively). Conclusion The allele HLA DQA1 0 30 1 may be a genetic predisposition to AP in Chinese Han children, which is involved in the pathogenesis of joint, gastrointestinal tract and kidney, whereas the DQA1 0 30 2 allele may be the gene of its genetic protection