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Based on the single-chain structure model of magnetorheological fluids, a formula for the calculation of shear stresses was established. The interaction force of two magnetic particles in an infinite single-chain was deduced using a new theoretical model which is founded on Ampere molecular current hypothesis, dipole theory and Ampere law. Furthermore, the resultant force on a particle was then deduced by taking into account of the action caused by all the other particles in the single-chain. A predictive formula for shear stresses was made corresponding to the case that MR fluids were sheared by a small angle and the calculating results fit well on the order with the yield stresses of the commercial MR fluids.