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目的 :探讨雌、孕激素和表皮生长因子与子宫肌瘤生长的关系。方法 :应用免疫组化ABC法检测 4 0例子宫肌瘤患者子宫组织雌激素受体 (ER)、孕激素受体 (PR)和表皮生长因子受体(EGFR)的表达 ,同时用磁性微粒载体免疫酶联法 (IEMA)测定血清雌二醇 (E2 )和孕酮 (P)水平 ;用酶联免疫吸附法 (EL ISA)测定血清表皮生长因子 (EGF)水平。结果 :子宫肌瘤组织 ER、EGFR表达显著高于子宫肌层组织 (P<0 .0 5 ,P<0 .0 1) ,子宫肌瘤组织 PR表达显著高于 ER表达 (P<0 .0 0 5 ) ,肌瘤组织和肌层组织分泌期 EGFR表达显著高于增生期 (P<0 .0 5 )。子宫肌瘤组分泌期血清 E2 、P水平显著高于对照组 (P<0 .0 5 ,P<0 .0 0 1) ,肌瘤组和对照组分泌期 EGF水平均明显高于增生期 (P<0 .0 0 1)。结论 :子宫肌瘤的生长与血清 E2 、P水平升高和子宫组织 ER、PR和 EGFR的表达增强有关。P可能诱导 EGF的分泌和 EGFR的表达
Objective: To investigate the relationship between estrogen, progesterone and epidermal growth factor and uterine fibroids growth. Methods: Immunohistochemical ABC method was used to detect the expression of estrogen receptor (ER), progesterone receptor (PR) and epidermal growth factor receptor (EGFR) in uterine tissue of 40 patients with uterine fibroids. At the same time, Serum estradiol (E2) and progesterone (P) levels were measured by enzyme-linked immunosorbent assay (IEMA). Serum epidermal growth factor (EGF) levels were measured by enzyme linked immunosorbent assay (ELISA) Results: The expression of ER and EGFR in uterine myoma was significantly higher than that in the myometrium (P <0.05, P <0.01). The expression of PR in uterine fibroids was significantly higher than that in ER (P <0. 0 0 5), the expression of EGFR in myometrium and muscular tissue during secretory phase was significantly higher than that in proliferative phase (P <0.05). The serum level of E2 and P in secretory phase of uterine fibroids was significantly higher than that in control group (P <0.05, P <0.01), and the secretion of EGF in fibroid group and control group was significantly higher than that in proliferative phase P <0 0 0 1). Conclusion: The growth of uterine fibroids is related to the increase of serum E2 and P levels and the expression of ER, PR and EGFR in uterus. P may induce EGF secretion and EGFR expression