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选用抗稻瘟病水稻品种沈农606为抗病亲本,以普感品种丽江新团黑谷为感病亲本配制杂交组合。对两亲本及其F2代单株进行苗期抗病鉴定,结果表明抗病亲本的抗性由核基因控制,受一对显性基因控制。根据F2代单株的抗病鉴定结果,采用BSA法,从110对SRAP引物中筛选出了4对在抗、感池间表现出多态性的引物,表明这些引物可能与沈农606的抗病基因连锁。利用这些标记对F2代112个感病单株进行扩增,根据扩增结果,采用Mapmaker 3.0软件计算遗传距离,结果显示,标记m5e1-500与抗病基因间的遗传距离最近,为2.8cM。
Shennong 606, an anti-rice blast resistant rice variety, was selected as the disease-resistant parent, and a hybrid combination was made with susceptible parents of susceptible variety Lijiang Xinqunhei Heigu. The results showed that the resistance of the resistant parents was controlled by nuclear genes and controlled by a pair of dominant genes. According to the resistance evaluation results of F2 plants, four pairs of primers were screened out from 110 pairs of SRAP primers using BSA method. The results showed that these primers may be related to the resistance of Shennong 606 Disease genes linked. Using these markers to amplify 112 susceptible plants in F2 generation, the genetic distance was calculated using Mapmaker 3.0 software based on the amplification results. The results showed that the genetic distance between the marker m5e1-500 and the resistant gene was 2.8 cM.