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目的：探讨EV71感染所致手足口病患儿外周血淋巴细胞亚群的变化及临床意义。方法将88例经粪便RT-PCR试验检测为EV71感染的手足口病患儿分为三组并收集血液标本，普通型24例，重症型21例，危重型43例。采用流式细胞术检测外周血C D3+T细胞、C D4+T细胞、C D8+T细胞、C D56+T细胞、CD19+T细胞表达。所有数据采用STATA软件进行统计。结果危重型患儿外周血CD3+T细胞比例为50.70±9.42%，重型组患儿外周血CD3+T细胞比例为50.43±10.89%，普通型组患儿外周血CD3+T细胞比例为55.57±9.76%，重型和危重型患儿外周血细胞CD3+T细胞比例较普通型组降低，但差别无显著统计学意义。（P>0.05)。危重型患儿外周血中CD4+T细胞比例为24.87±7.78%，重型组患儿外周血CD4+T细胞比例为25.06±7.87%，普通型患儿外周血CD4+细胞比例30.18±7.18%，危重型组和重型组患儿外周血C D4+T细胞比例较普通型组降低，且随病情加重而减低。三组患儿C D8+、C D56+、C D19+T细胞比例无显著差异（P>0.05)。结论 T淋巴细胞减少可能是EV71感染手足口病患儿并发重症和危重症的重要因素之一。“,”Objective: To analyze the changes in peripheral blood T-lymphocytes subpopulation in children with hand foot mouth disease infected by enterovirus 71.Methods:The blood samples from Children who confirmed infected by enterovirus -71 were col ected and divided into three groups:24 patients without complications,21patients with central nervous system involvment ,43 patients with autonomic nervous system dysregulation (among them two patients with PE).The level of CD3+, CD4+, CD8+, CD56+, CD19+ and CD4/CD8 were detected by flow cytometry(FCM).RESULTS: The levels of CD3+ and CD4+ in severe cases were lower than that in light cases.There were no significant difference of CD8+, CD56+, CD19+ in three groups.Conclusion:The T-lymphocyte decrease play a important role in the formation of severe cases.