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以鼠曲草为试材,通过单因素试验和正交实验,研究了超声提取野生鼠曲草中水溶性黄酮类化合物和多糖的最佳工艺条件。结果表明:黄酮类化合物和多糖的最佳工艺分别为以蒸馏水为提取剂,提取时间50min、提取温度80℃、超声功率160W、料液比1∶50g/mL和提取时间50min、提取温度75℃、超声功率160W、料液比1∶60g/mL;此条件下提取黄酮类化合物和多糖耗样少,快速准确,水溶性的黄酮类化合物和多糖的提取率分别为4.36%和3.09%。该法类黄酮加标平均回收率为96.96%,RSD<3%;还原糖和总糖平均加标回收率为98.08%和101.8%。
In this paper, we studied the optimal conditions for the ultrasonic extraction of water-soluble flavonoids and polysaccharides from wild kurdis by single factor test and orthogonal experiment. The results showed that the optimum conditions of flavonoids and polysaccharides were distilled water as extraction agent, extraction time 50min, extraction temperature 80 ℃, ultrasonic power 160W, solid-liquid ratio 1:50g / mL and extraction time 50min, extraction temperature 75 ℃ , Ultrasonic power of 160W and ratio of solid to liquid of 1:60 g / mL. Under these conditions, the extraction rates of flavonoids and polysaccharides with less flavonoids and polysaccharides were 4.36% and 3.09%, respectively. The average flavonoid flavonoid recovery was 96.96% with RSD <3%. The average recoveries of reducing sugar and total sugar were 98.08% and 101.8%, respectively.