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目的探讨胎膜早破及其绒毛羊膜炎孕妇血和羊水中细胞因子的变化。方法采用放射免疫法和酶联免疫吸附试验对４６例胎膜早破孕妇和２０例正常足月孕妇血及羊水中白细胞介素８（ＩＬ８）、肿瘤坏死因子（ＴＮＦ）α水平进行测定。结果胎膜早破孕妇血中ＩＬ８和羊水中ＩＬ８、ＴＮＦα水平均明显高于对照组（Ｐ＜０．０１～０．０５）；破膜时间与母血中ＩＬ８、羊水中ＩＬ８、ＴＮＦα水平有明显关系，破膜时间越长母血中ＩＬ８、羊水中ＩＬ８、ＴＮＦα水平越高；绒毛羊膜炎孕妇血和羊水中ＩＬ８、ＴＮＦα水平均明显高于非绒毛羊膜炎孕妇（Ｐ＜０．０１～０．０５）。结论母血和羊水中ＩＬ８、ＴＮＦα水平可以作为识别胎膜早破和绒毛羊膜炎的一个标志。
Objective To investigate the changes of cytokines in blood and amniotic fluid of premature rupture of membranes and their chorioamnionitis. Methods Radioimmunoassay and enzyme-linked immunosorbent assay were used to determine the levels of interleukin 8 (IL 8) and tumor necrosis factor (TNF) α in 46 cases of premature rupture of membranes and 20 cases of normal term pregnant women. Results The blood levels of IL8 and amniotic fluid IL8 and TNFα in pregnant women with premature rupture of membranes were significantly higher than those in control group (P <0.01 ~ 0.05). The time of rupture of membranes and IL8 in serum and IL8 and TNFα in amniotic fluid were Significant relationship between the rupture time IL8, amniotic fluid levels of IL8, TNFα higher; chorioamnionitis serum levels of amniotic fluid and amniotic fluid IL8, TNFα levels were significantly higher than non-chorioamnionitis pregnant women (P <0.01 ~ 0.05). Conclusion The levels of IL8 and TNFα in maternal blood and amniotic fluid can be used as a marker to identify premature rupture of membranes and chorioamnionitis.