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目的检测基质金属蛋白酶13(MMP-13)和组织金属蛋白酶抑制因子1(TIMP-1)的血清含量,分析其在妇女绝经后骨质疏松发病中的作用。方法 2009年3月-2012年9月选取武汉附近地区129例49~63岁绝经后妇女,根据双能X线吸收法检测的骨密度数值,分为正常组、低骨量组和骨质疏松组。采取酶联免疫吸附试验检测MMP-13、TIMP-1以及雌二醇(E2)、Ⅰ型原胶原N端前肽(PINP)和Ⅰ型胶原交联C末端肽(CTX)、骨保护蛋白(OPG)及其配体(OPGL)的含量,统计MMP-13/TIMP-1比值。结果①骨质疏松组中血清MMP-13水平[(44.25±1.21)μg/L]高于正常组[(27.08±1.41)μg/L](P<0.05);②骨质疏松组中血清MMP-13与骨密度、血清E2、OPGL水平存在明显负相关性(P<0.05),和OPG、PINP和CTX存在明显正相关性(P<0.05);③低骨量组中MMP-13略高于骨质疏松组,且两者差异无统计学意义(P>0.05),但是明显高于正常组(P<0.05),同时与骨密度和血清E2、OPG、OPGL、PINP和CTX存在明显相关性(P<0.05)。结论血清MMP-13和MMP-13/TIMP-1比值与绝经后骨质疏松症妇女和绝经后低骨量组妇女骨代谢指标具有关联性。两者升高可能为绝经后妇女早期骨代谢尤其是胶原代谢过程增快的表现。
Objective To detect the serum levels of matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in women with postmenopausal osteoporosis. Methods From March 2009 to September 2012, 129 cases of postmenopausal women aged 49-63 years in the vicinity of Wuhan were selected and divided into normal group, low bone mass group and osteoporosis group according to the BMD measured by dual energy X-ray absorption method group. The levels of MMP-13, TIMP-1 and E2, type Ⅰ procollagen N-terminal propeptide (PINP) and type Ⅰ collagen C-terminal peptide (CTX) were detected by enzyme linked immunosorbent assay OPG) and its ligand (OPGL) content, statistical MMP-13 / TIMP-1 ratio. Results ① The serum levels of MMP-13 in the osteoporosis group were significantly higher than those in the normal group [(44.25 ± 1.21) μg / L [(27.08 ± 1.41) μg / L, P <0.05] (P <0.05). There was a significant positive correlation between OPG, PINP and CTX (P <0.05). ③The MMP-13 in low bone mass group was slightly higher than that in OPG, PINP and CTX OPG, OPGL, PINP and CTX in osteoporosis group were significantly higher than those in normal group (P <0.05), but there was no significant difference between the two groups (P> 0.05) (P <0.05). Conclusions The serum MMP-13 and MMP-13 / TIMP-1 ratios are correlated with the bone metabolic parameters in postmenopausal women and postmenopausal women with low bone mass. Elevated both may be postmenopausal women early bone metabolism, especially collagen metabolism faster performance.