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目的建立苏丹红I(Sudan I)免疫学检测方法,制备具有免疫原性的苏丹红I人工抗原。方法采用琥珀酸酐法合成苏丹红I的衍生物苏丹红I-半琥珀酸酯(苏丹红I-HS),在此基础上,采用活性酯法将苏丹红I-HS分别与牛血清白蛋白(BSA)和卵清蛋白(OVA)偶联制备得到苏丹红I-HS-BSA和苏丹红I-HS-OVA。结果采用红外光谱、液质联用对苏丹红I-HS进行分析和鉴定表明合成成功,并用紫外光谱法测定苏丹红I与BSA和OVA偶联比分别为11∶1和5∶1。结论苏丹红I人工抗原合成成功。
Objective To establish a Sudan I immunoassay for the preparation of immunogenic Sudan I artificial antigen. Methods Sudan I-hemisuccinate (Sudan I-HS), a derivative of Sudan I, was synthesized by the succinic anhydride method. Based on this, Sudan I-HS was mixed with bovine serum albumin BSA) and ovalbumin (OVA) to prepare Sudan I-HS-BSA and Sudan I-HS-OVA. Results The analysis and identification of Sudan I-HS by IR and LC-MS showed successful synthesis. The coupling ratio of Sudan I to BSA and OVA was 11:1 and 5:1, respectively. Conclusion Sudan I artificial antigen synthesis success.