论文部分内容阅读
采用酸性聚丙烯酰胺凝胶电泳（ＡＰＡＧＥ） 对四川省近５０ 年来年推广面积６６６ 万ｈｍ２ 以上的４０ 个小麦主栽品种进行醇溶蛋白位点特异性检测，分析了不同基因型间醇溶蛋白的遗传差异。结果表明：４０ 个品种具有３８ 种醇溶蛋白带型，其中９ 个品种具有１ＢＬ／１ＲＳ易位标记位点ＧｌｉＢｌｌ；醇溶蛋白电泳分离出的４６ 条带中，４０ 条具有多态性，占８４８ ％ 。供试品种间遗传距离（ＧＤ）在０ ～０６７ 之间，平均值为０３３ ，遗传变异较大，特别是早期品种与近２０ 年育成品种间，以及１ＢＬ／１ＲＳ易位系品种与非１ＢＬ／１ＲＳ易位系品种间均具有较大的遗传距离。聚类分析表明：供试品种在遗传距离０３５ 水平上明显聚为４ 类，具有相同血缘的品种大多数聚在了同１ 类。分析认为，四川小麦品种醇溶蛋白变异丰富，存在广泛的遗传多样性
The 40 loci of gliadin were detected by acid polyacrylamide gel electrophoresis (APAGE) in 40 wheat cultivars with the popularized area of 66,600 hm2 over the past 50 years in Sichuan Province. The effects of different genotypes of alcohol Genetic differences in proteolysin. The results showed that there were 38 gliadin bands in 40 cultivars, among which 9 cultivars had 1BL / 1RS translocation marker site GliBll, and of the 46 bands isolated by gliadin electrophoresis, 40 were polymorphic , Accounting for 84 8%. The genetic distance (GD) between the tested cultivars ranged from 0 to 067, with an average of 0.33. The genetic variation was large, especially between the early cultivars and the cultivars cultivated in the past 20 years, as well as the 1BL / 1RS translocation line With non-1BL / 1RS translocation lines have a greater genetic distance between. Cluster analysis showed that the tested cultivars were obviously clustered into 4 categories at the genetic distance of 035 and most of the cultivars with the same relatives clustered in the same category. Analysis, Sichuan varieties of gliadin variants rich in genetic diversity