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目的:分析二期梅毒患者外周血单个核细胞基因表达谱特征,探索机体抗梅毒免疫的分子机制。方法:采用全基因组高通量的Illumina测序技术,对4例二期梅毒患者和4例健康对照者外周血单个核细胞行数字基因转录组分析。后行实时PCR对其结果进行验证。结果:与健康对照者相比较,二期梅毒患者外周血单个核细胞共发现差异表达基因78个,其中有16个免疫应答相关基因。肿瘤坏死因子超家族成员17(TNFRSF17)、IL-17C、IL-21、IL-31受体A(IL-31RA)、C-X-C配体10(CXCL10)、趋化因子配体1(CCL1)等炎症因子和相关受体、CD4+T细胞激活标志CD38、Fc类吞噬性受体(FcγR1A、FcγR3B)以及补体(C2、SERPING1)等均显著上调。结论:二期梅毒患者多种天然免疫和适应性免疫分子参与机体系统性抗梅毒应答。
Objective: To analyze the gene expression profiles of peripheral blood mononuclear cells in patients with secondary syphilis and to explore the molecular mechanism of anti-syphilis immunity. Methods: Genome-wide high-throughput Illumina sequencing was used to analyze the gene transcriptome of peripheral blood mononuclear cells in 4 patients with secondary syphilis and 4 healthy controls. After real-time PCR to verify the results. Results: Compared with healthy controls, 78 differentially expressed genes were found in peripheral blood mononuclear cells of patients with secondary syphilis, of which 16 were related to immune response. Tumor necrosis factor superfamily member 17 (TNFRSF17), IL-17C, IL-21, IL-31RA receptor A (IL-31RA), CXC ligand 10 (CXCL10), chemokine ligand 1 (CCL1) CD4 + T cell activation marker CD38, Fc-like phagocytic receptors (FcγR1A, FcγR3B) and complement (C2, SERPING1) were all significantly up-regulated. CONCLUSIONS: A variety of innate and adaptive immune molecules in patients with secondary syphilis are involved in the systemic anti-syphilis response.