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应用流式细胞术和抗Ｋｉ－６７单克隆抗体检测了４０例小儿急性白血病外周血白血病细胞的增殖情况，其中未经治疗的急性白血病３０例，完全缓解者１０例。结果表明：未经治疗的患儿Ｋｉ－６７表达值明显高于完全缓解组和正常对照组（Ｐ＜０．００５）。急性非淋巴细胞白血病组Ｋｉ－６７表达值高于急性淋巴细胞白血病组（Ｐ＜０．０５），Ｋｉ－６７表达值与流式细胞术检测的Ｓ期百分数成正相关。Ｋｉ－６７表达与ＤＮＡ倍体有关，ＤＮＡ异倍体组Ｋｉ－６７表达值高于ＤＮＡ二倍体组（Ｐ＜０．０５）。
Flow cytometry and anti-Ki-67 monoclonal antibody detection of 40 cases of pediatric acute leukemia peripheral blood leukemia cell proliferation, including 30 cases of untreated acute leukemia, complete remission in 10 cases. The results showed that: Ki-67 expression in untreated children was significantly higher than the complete remission group and the normal control group (P <0.005). The expression of Ki-67 in acute non-lymphocytic leukemia group was higher than that in acute lymphoblastic leukemia group (P <0.05). Ki-67 expression was positively correlated with the percentage of S phase detected by flow cytometry. The expression of Ki-67 was related to DNA ploidy. The expression of Ki-67 in DNA aneuploidy group was higher than that in DNA diploid group (P <0.05).