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目的检测肺癌组织中脆性组氨酸三聚体(Fragile Histidine Triad,FHIT)和锌指结构转录抑制因子Snail的表达以及两者与临床病理特征的关系。方法采用免疫组织化学方法检测80例肺癌及相应癌旁正常肺组织中FHIT和Snail蛋白的表达情况。结果在肺癌中FHIT的阳性表达率为31.25%(25/80),低于癌旁正常肺组织71.25%(57/80),两者比较差异有统计学意义(P<0.05);在肺癌中Snail的阳性表达率为76.25%(61/80),高于癌旁正常肺组织32.50%(26/80),两者比较差异有统计学意义(P<0.05);FHIT的阳性表达率与患者吸烟史、肿瘤的病理类型、分化程度、p TNM分期、淋巴结转移及术后生存时间有关(P<0.05)。Snail与肿瘤的分化程度、TNM分期、淋巴结转移及术后生存时间有关(P<0.05);Snail蛋白的表达水平与FHIT呈负相关(P<0.05)。结论 Snail高表达与FHIT低表达与肺癌的侵袭、转移密切相关。
Objective To detect the expression of Fragile Histidine Triad (FHIT) and Snail, a transcriptional repressor of zinc finger, in lung cancer and their relationship with clinicopathological features. Methods Immunohistochemistry was used to detect the expression of FHIT and Snail protein in 80 cases of lung cancer and corresponding normal lung tissues. Results The positive expression rate of FHIT in lung cancer was 31.25% (25/80), which was lower than 71.25% (57/80) in the normal lung tissue adjacent to cancer. The difference was statistically significant (P <0.05). In lung cancer The positive rate of Snail was 76.25% (61/80), which was significantly higher than that of normal lung tissue (32.50%, 26/80) (P <0.05) Smoking history, tumor pathological type, differentiation, p TNM stage, lymph node metastasis and postoperative survival time (P <0.05). Snail was correlated with tumor differentiation, TNM stage, lymph node metastasis and postoperative survival time (P <0.05). Snail protein expression was negatively correlated with FHIT (P <0.05). Conclusion The high expression of Snail and the low expression of FHIT are closely related to the invasion and metastasis of lung cancer.