论文部分内容阅读
目的：建立改良的人胰蛋白酶Ⅰ放射免疫测定（ＨＰＥ１ＲＩＡ）法并观察此法对临床诊断胰腺癌的价值。方法：纯化ＨＰＥ１，制备兔抗人ＨＰＥ１ＩｇＧ，在此基础上建立改良ＨＰＥ１ＲＩＡ法，临床测定了８２例健康成人和２２２例各类疾病病人血清ＨＰＥ１值。结果：８２例健康成人血清ＨＰＥ１值为７３～３２０ｎｇ／ｄｌ，９３例胰腺癌患者血清ＨＰＥ１值为８３３±７３４ｎｇ／ｄｌ（ｘ±ｓ）。在胰腺癌患者中，８例肿瘤直径小于３ｃｍ的胰头癌ＨＰＥ１值（１２２５±１２１８ｎｇ／ｄｌ）明显高于胰腺癌各分组中任何一组的血清ＨＰＥ１值（Ｐ＜０．０１）。结论：改良ＨＰＥ１ＲＩＡ法可作为胰腺癌诊断筛选的一种有价值的新方法。
Objective: To establish a modified human trypsin I radioimmunoassay (HPE1RIA) method and observe its value in clinical diagnosis of pancreatic cancer. METHODS: HPE1 was purified to prepare rabbit anti-human HPE1 IgG. Based on this, a modified HPE1 RIA method was established. Serum HPE1 values were determined in 82 healthy adults and 222 patients with various diseases. Results: The serum HPE1 value of 82 healthy adults was 73-320 ng/dl, and the serum HPE1 value of 93 patients with pancreatic cancer was 833±734 ng/dl (x±s). Among the patients with pancreatic cancer, the HPE1 value (1225±1218 ng/dl) was significantly higher in 8 pancreatic head cancers with a tumor diameter less than 3 cm than in any group of pancreatic cancer (P<0.01). Conclusion: The modified HPE1RIA method can be used as a valuable new method for diagnosis and screening of pancreatic cancer.