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AIM: To study the effects of phosphorus-32 glass microspheres(~(32)P-GMS) on human hepatocellular carcinoma in nude mice.METHODS: Human liver cancer cell line was implanted intothe dorsal subcutaneous tissue of 40 BALB/c nude mice.Then the 40 tumor-bearing BALB/c nude mice were allocatedinto treatment group (n=32) and control group (n=8).Inthe former group different doses of ~(32)P-GMS were injectedinto the tumor mass,while in the latter nonradioactive ~(31)P-GMS was injected into the tumor mass.The experimentalanimals were sacrificed on the 14th day.The ultrastructuralchanges of tumor in both treatment group and control groupwere studied with transmission electron microscopy (TEM)and stereology.RESULTS: In treatment group,a lot of tumor cells werekilled and the death rate of tumor cells was much higher(35-70%).UItrastructurally,severe nuclear damage wasobserved in the death cells.The characteristics of appoptosissuch as margination of heterochromatin was also found insome tumor cells.Besides,well differentiated tumor cells,degenerative tumor cells and some lymphocytes were seen.The skin and muscle adjacent to the tumor were normal.Incontrol group,the tumor consisted of poorly differentiatedtumor cells,in which there were only a few of dead cells(5%).Stereologicl analysis of ultrastructral morphologyshowed that Vv of nuclei (53.31±3.46) and Vv of nucleoli(20.40±1.84) in the control group were larger than those(30.21±3.52 and 10.96±2.52) in the treatment grouprespectively (P<0.01),and Vv of RER (3.21±0.54) and Vv ofmitochondria (4.53±0.89) in the control group were smallerthan those (8.67±1.25 and 7.12±0.95) in the treabment grouprespectively (P<0.01,0.05).Sv of the membrane of microvilliand canaliculi (27.12 um~2/100 um~3±11.84 um~2/100 um~3) in thecontrol group was smaller than that (78.81 um~2/100 um~3±19.69 um~2/100 um~3) in the treatment group (P<0.01).But Wof lipid particles (3.71±1.97) and Vv of vacuoles (5.72±1.58)were much larger than those (0.30±0.16 and 0.35±0.15) inthe treatment group respectively (P<0.05,P<0.01).CONCLUSION: The experimental results indicate that localadministration of ~(32)p-GMS can produce obvious effect on liver cancer cells and the anticancer effect of ~(32)P-GMS isdirectly proportional to the dose administrated.Ultrastructuralstereology can also show the effect of ~(32)P-GMS on thenormalization of tumor cells,which is beneficial to theprognosis and treatment of patients.Moreover,localadministrtion of ~(32)p-GIVlS is also safe.
AIM: To study the effects of phosphorus-32 glass microspheres (~ (32) P-GMS) on human hepatocellular carcinoma in nude mice. METHODS: Human liver cancer cell line was implanted intothe dorsal subcutaneous tissue of 40 BALB / c nude mice. Inthe former group different doses of ~ (32) P-GMS were injected in the tumor mass, while in the latter nonradioactive ~ (31) P-GMS was injected into the tumor mass. The experimentally animals were sacrificed on the 14th day. ultrastructuralchanges of tumor in both treatment group and control groupwere studied with transmission electron microscopy (TEM) and stereology .RESULTS: In treatment group, a lot of tumor cells werekilled and the death rate of tumor cells was much higher (35-70%). UItrastructurally, severe nuclear damage wasobserved in the death cells. Characteristics of appoptosissuch as margination of heterochromatin was also found insome tumor cells. In addition, well differentiated tumor cells, degenerative tumor cells and some lymphocytes were seen. The skin and muscle adjacent to the tumor were normal. Incontrol group, the tumor consisted of poorly differentiated tumor cells, in there there were only a few of dead cells (5 %). Stereologicl analysis of ultrastructural morphologic changes in Vv of nuclei (53.31 ± 3.46) and Vv of nucleoli (20.40 ± 1.84) in the control group were larger than those (30.21 ± 3.52 and 10.96 ± 2.52) in the treatment group separately (P < 0.01), and Vv of RER (3.21 ± 0.54) and Vv of mitochondria (4.53 ± 0.89) in the control group were smallerthan those (8.67 ± 1.25 and 7.12 ± 0.95) in the trembment group separately (P <0.01, 0.05) the membrane of microvilliand canaliculi (27.12 um ~ 2/100 um ~ 3 ± 11.84 um ~ 2/100 um ~ 3) in the control group was smaller than that (78.81 um ~ 2/100 um ~ 3 ± 19.69 um ~ 2/100 um ~ 3 in the treatment group (P <0.01). But Wof lipid particles (3.71 ± 1.97) and Vv of vacuoles (5.72 ± 1.58) were much larger than those (0.30 ± 0.16 and 0.35 ± 0.15) inthe treatment group respectively (P <0.05, P <0.01) .CONCLUSION: The experimental results indicate that localadministration of ~ (32) p-GMS can produce obvious effect on liver cancer cells and the anticancer effect of ~ ( 32) P-GMS is directly proportional to the dose administered. Ultrastructuralstereology can also show the effect of ~ (32) P-GMS on thenormalization of tumor cells, which is beneficial to the prognosis and treatment of patients. Moreover, localadministration of ~ (32) p-GIVlS is also safe.