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目的观察诱导后的成骨细胞在Mg-Mn-Zn表面附着与增殖情况。方法分离培养兔骨髓基质干细胞(BMSCs),将其诱导分化为成骨细胞后,以1×108/L的细胞浓度种植在Mg-Mn-Zn及对照组纯钛表面,联合培养1、3、5d后,分别用扫描电镜、激光扫描共焦显微镜观察两种金属表面的细胞形态学变化及生长情况。结果 BMSCs以球状体方式进行克隆生长,经诱导后的细胞表达成骨细胞特性,在与镁合金复合培养后,细胞发生黏附并增殖连接成片。结论诱导后的成骨细胞在Mg-Mn-Zn表面有较好的分裂增殖能力,提示镁合金有较好的骨诱导生成作用。
Objective To observe the attachment and proliferation of osteoblasts on Mg-Mn-Zn surface. Methods Rabbit bone marrow stromal cells (BMSCs) were isolated and cultured and differentiated into osteoblasts. The cells were seeded on Mg-Mn-Zn and control titanium surfaces at the concentration of 1 × 108 / L. After 5 days, morphological changes and growth of the two metal surfaces were observed by scanning electron microscopy and laser scanning confocal microscopy. Results BMSCs were cloned by spheroid. The induced cells expressed osteoblasts. After being cultured with magnesium alloy, BMSCs adhered and proliferated into pieces. Conclusions The induced osteoblasts have better ability to divide and proliferate on the surface of Mg-Mn-Zn, which implies that magnesium alloy has good osteoinductive effect.