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本研究以紫花苜蓿为实验对象,以采煤沉陷区退化土壤为培养基质,通过人为伤根模拟矿区沉陷对根系造成的损伤,采用量化模拟方法分析了丛枝菌根真菌在减缓伤根中所起的作用。结果表明,在剪切紫花苜蓿根系后,接菌处理地上干重和根系干重均高于对照处理,在剪切根系程度较小时(1/3和1/2)苜蓿-菌根共生体能够缓解对根系的损伤,但是,剪切根系较大时(2/3和3/4)菌根效应降低,对植物生长发育促进作用减小。接菌改善了紫花苜蓿根际微环境,缓解了根系损伤对植物的负面作用。
In this study, alfalfa was used as the experimental object, with the degraded soil in the mining subsidence area as the culture substrate. The damage caused by the subsidence of the mining area to the root system was simulated by artificial wound root. The quantitative simulation method was used to analyze the effects of arbuscular mycorrhizal fungi Play a role. The results showed that after cutting the roots of alfalfa, the aboveground dry weight and root dry weight of the inoculation treatments were higher than those of the control treatments. When the roots were cut to a small extent (1/3 and 1/2), the alfalfa - mycorrhizae symbionts could Alleviate the damage to the root system, however, when the root system is sheared, the mycorrhizal effect is reduced (2/3 and 3/4), and the effect on plant growth and development is reduced. Inoculation improved alfalfa rhizosphere microenvironment and alleviated the negative effects of root damage on plants.