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本文建立了高效液相色谱法测定人血清中的对乙酰氨基酚浓度，结果表明该方法精密度高，灵敏度好，线性关系良好，ｒ＝０．９９９８（ｎ＝６），最低检测浓度为０．１ｍｇ／ｌ，平均日内和日间变异系数＜９％。８名健康志愿者交叉口服对乙酰氨基酚片和对乙酰氨基酚ＰＶＰ片，药代动力学参数分别为，Ｋａ：１．８６±０．９５，３．２３±２．６０１／ｈ；Ｔ１／２：３．１７±１．１３，２．７０±０．８５ｈ；Ｃｍａｘ：７．５６±２．０８，８．２４±１．３７ｍｇ／ｌ；Ｔｐｋ：１．０６±０．３９，０．７９±０．３０ｈ，ＡＵＣ：３２．９１±８．４５，３４．２５±８．４５ｍｇ·ｈ~（－１）·ｌ~（－１）。两种片剂的药代动力学参数（除Ｋａ值外）均无显著性差异，Ｐ＞０．０５。其中对乙酰氨基酚ＰＶＰ片吸收明显快于对乙酰氨基酚（Ｐ＜０．０５）。平均生物利用度为１０４．０７％，两种片剂生物等效。
In this paper, HPLC was established for the determination of paracetamol in human serum. The results showed that the method has high precision, good sensitivity and good linearity (r = 0.9998 (n = 6), the lowest detection concentration is 0 .1mg / l, the average daily and intra-day coefficient of variation <9%. Pharmacokinetic parameters of paracetamol tablets and paracetamol PVP tablets were respectively taken orally by eight healthy volunteers. The Ka: 1.86 ± 0.95 and 3.23 ± 2.601 / h, T1 / 2: 3.17 ± 1.13, 2.70 ± 0.85 h; Cmax: 7.56 ± 2.08, 8.24 ± 1.37 mg / l; Tpk: 1.06 ± 0.39, 79 ± 0.30h, AUC: 32.91 ± 8.45,34.25 ± 8.45mg · h -1 · -1. Pharmacokinetic parameters (except Ka value) of the two tablets showed no significant difference, P> 0.05. Paracetamol PVP tablet absorption was significantly faster than acetaminophen (P <0.05). The average bioavailability was 104.07%, and both tablets were bioequivalent.