论文部分内容阅读
聚合酶链式反应(Potgnerase Chain Reation,PCR)由美国PE公司遗传部的Mullis于1984年发明建立,Mullis因此而获93年度诺贝尔化学奖;1985年,Sairi等人首先于《Science》杂志上完整地描述了该反应,1987年美国专利局授予PCR技术专利。1988年,Mullis等人又从黄石公园温泉中分离出嗜热菌Thermus aouaticus并从中提取Tag DNA聚合酶,该酶能耐受95℃高温而不失活,它替代热不稳定的大肠肝菌DNA聚合酶,使PCR技术变得简便易行,PCR技术从此飞速发展,对现代生物学研究起了强大的推进作用。
Polyglutamic chain reaction (Potgnerase Chain Reation, PCR) by the United States PE company Mullis Department of Genetics invented in 1984, Mullis thus won the 93 annual Nobel Prize in Chemistry; 1985, Sairi et al first in the “Science” magazine The reaction is fully described, and in 1987 the U.S. Patent Office granted the patent for PCR technology. In 1988, Mullis et al. Again isolated the thermophilic bacterium Thermus aouaticus from Yellowstone Hot Springs and extracted Tag DNA polymerase, which is resistant to high temperatures of 95 ° C and replaces heat-labile E. coli DNA Polymerase makes PCR technology simple and easy. The rapid development of PCR technology has played a significant role in promoting modern biology.