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本研究旨在探讨叶酸聚谷氨酸合成酶(FPGS)基因中rs10760502G>A多态性与儿童急性B淋巴细胞白血病(B-ALL)预后及甲氨蝶呤(MTX)毒副反应的相关性。采用Sequenom MassARRAY时间飞行质谱系统检测rs10760502基因型。实验数据采用x2检验、Kaplan-Meier生存分析、Cox回归分析等统计方法处理。结果表明:A等位基因携带者(GA+AA)无复发生存率(RFS,log-rank:P=0.004)及无事件生存率(EFS,log-rank:P=0.022)显著低于GG等位基因型携带者。多因素Cox回归分析显示,A等位基因是RFS[hazard ratio(HR),20.173;95%CI,2.535-160.545;P=0.005]及EFS(HR,8.133;95%CI,1.718-38.512;P=0.008)的独立不良预后因素。rs10760502多态与MTX毒副反应间未见相关性。结论:FPGS rs10760502G>A多态性位点可能作为BALL患儿的独立预后因素。
This study aimed to investigate the association of rs10760502G> A polymorphism in folic acid polyglutamate (FPGS) gene with the prognosis of children with acute B-cell lymphocytic leukemia (B-ALL) and methotrexate (MTX) toxicity . The rs10760502 genotype was detected using the Sequenom MassARRAY time-of-flight mass spectrometry system. The experimental data using x2 test, Kaplan-Meier survival analysis, Cox regression analysis and other statistical methods. The results showed that the relapse-free survival rate (RFS, P-0.004) and event-free survival (EFS, log-rank: P = 0.022) of A allele carriers were significantly lower than those of GG patients Genotype carrier. Multivariate Cox regression analysis showed that the allele A was a hazard ratio (HR) of 20.173; 95% CI 2.535-160.545; P = 0.005] and EFS (HR, 8.133; 95% CI, 1.718-38.512; P = 0.008) independent adverse prognostic factors. There was no correlation between rs10760502 polymorphism and MTX toxicities. Conclusion: The FPGS rs10760502G> A polymorphism may be an independent prognostic factor in children with BALL.