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确定苍蝇保持传染性的时间是重要的。据文献介绍,在苍蝇体表的营养型细菌在数小时或几天内死亡,其流行病学意义不大,一般认为霍乱弧菌在苍蝇体内存活时间不超过4—5天。本研究用于苍蝇实验性感染的霍乱弧菌系生长于营养丰富的固体培养基(densefeed medium)37℃18—20小时。苍蝇用羽化后第2至第3天的。在感染前苍蝇不给水和食物12—18小时,然后喂给含霍乱病原体的液体。整个实验过程中苍蝇被隔离并饲以30%全乳粉状糊(mealy Jelly),每次细菌学检查用3—5只苍蝇,在研钵内研碎,悬浮于5毫升灭菌生理盐水中,然后接种于1%硷性蛋白胨水中孵育37℃6—8小时,再接种于固体培养基分离霍乱弧菌菌
It is important to determine when flies stay contagious. According to the literature, vegetative bacteria in flies die within a few hours or days, and their epidemiological significance is not significant. Vibrio cholerae are generally believed to survive in flies for no more than 4-5 days. In this study, Vibrio cholerae strains used for experimentally infected flies were grown in a densefeed medium at 37 ° C for 18-20 hours. Flies with the 2nd to 3rd days after eclosion. The flies do not feed water and food for 12-18 hours prior to infection, and then feed the fluids containing the cholera pathogens. The flies were quarantined and fed with 30% mealy Jelly throughout the experiment, 3-5 flies for each bacteriological examination, ground in a mortar and suspended in 5 ml sterilized saline , And then inoculated in 1% alkaline peptone water incubated 37 ℃ 6-8 hours, then inoculated on solid medium Vibrio cholerae