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目的:研究醋白芍、炙甘草及其配伍载体中醋白芍及炙甘草药效组分的变化规律。方法:以醋白芍、炙甘草单味药及其药对为研究对象,采用水煎煮法制备供试品溶液,采用HPLC法测定。结果:醋白芍4种药效组分(氧化芍药苷、芍药内酯苷、芍药苷、苯甲酰芍药苷)含量分别为:醋白芍(1.07±0.12、0.36±0.02、2.00±0.21、0.37±0.03)mg/m L;醋白芍-甘草(1.89±0.11、0.86±0.05、3.25±0.13、0.52±0.03)mg/m L;醋白芍-炙甘草(1.90±0.09、0.87±0.04、3.30±0.24、0.52±0.01)mg/m L。炙甘草五种药效组分(甘草酸、甘草苷、甘草素、异甘草苷、异甘草素)含量分别为:炙甘草(6.53±0.25、2.17±0.33、0.52±0.21、0.48±0.23、0.09±0.03、9.79±0.65)mg/m L;炙甘草-焦白芍(5.13±0.32、2.11±0.21、0.47±0.12、0.57±0.22、0.12±0.02、8.40±0.75)mg/m L;炙甘草-炒白芍(6.47±0.33、2.67±0.23、0.56±0.23、0.60±0.23、0.12±0.01、10.42±0.78)mg/m L;炙甘草-醋白芍(8.76±0.35、3.10±0.43、0.79±0.32、0.76±0.34、0.15±0.05、13.56±0.99)mg/m L。结论:配伍影响或改变了药效组分的物理、化学性质,进而改变了药效组分的溶出,最终改变了药效组分在复方汤剂中存在状态。中药治疗作用的关键在于药效组分,包括药效组分的组成及各药效组分之间的比例。药效组分的组成不同,疗效也不同;药效组分的组成相同,但比例不同,疗效也不一样。不同组成的药效组分或不同比例的药效组分分别代表不同的中药,具有不同的临床疗效,其根源在于药效组分的不同,应加以区分。因此,对中药药效组分的研究与探讨是解析中药配伍机制的关键。
OBJECTIVE: To study the changes of the pharmacodynamic components of vinegar peony and Zhigancao in vinegar peony root, Zhigancao and their compatible carriers. Methods: The vinegar peony root, Zhigancao single herb and its drug as the research object, the decoction method was used to prepare the test solution, determined by HPLC method. Results: The contents of four pharmacodynamic components (paeoniflorin, paeoniflorin, paeoniflorin and paeoniflorin) of vinegar peony root were 1.07 ± 0.12,0.36 ± 0.02,2.00 ± 0.21, 0.37 ± 0.03) mg / m L; vinegar Radix Paeoniae Alba - licorice root (1.89 ± 0.11,0.86 ± 0.05,3.25 ± 0.13,0.52 ± 0.03) mg / m L; vinegar Radix Paeoniae - licorice (1.90 ± 0.09,0.87 ± 0.04 , 3.30 ± 0.24, 0.52 ± 0.01) mg / m L. The contents of five medicinal components (glycyrrhizin, glycyrrhizin, licorice, isoliquiritigenin and isoliquiritigenin) of Zhigancao were: Zhigancao (6.53 ± 0.25, 2.17 ± 0.33, 0.52 ± 0.21, 0.48 ± 0.23, 0.09 ± 0.03,9.79 ± 0.65) mg / m L; Zhigancao - Jiao Baisha (5.13 ± 0.32,2.11 ± 0.21,0.47 ± 0.12,0.57 ± 0.22,0.12 ± 0.02,8.40 ± 0.75) mg / m L; Zhigancao - fried white peony root (6.47 ± 0.33,2.67 ± 0.23,0.56 ± 0.23,0.60 ± 0.23,0.12 ± 0.01,10.42 ± 0.78) mg / m L; Zhigancao - vinegar white peony root (8.76 ± 0.35,3.10 ± 0.43,0.79 ± 0.32, 0.76 ± 0.34, 0.15 ± 0.05, 13.56 ± 0.99) mg / m L. Conclusion: Compatibility affects or changes the physical and chemical properties of the medicinal components, thereby changing the dissolution of the medicinal components, and finally changing the existing state of the medicinal components in the decoction. The key to the therapeutic effect of traditional Chinese medicine lies in the pharmacodynamic components, including the composition of the medicinal components and the ratio between the medicinal components. Efficacy of the composition of different components, the curative effect is also different; the composition of the pharmacodynamic components of the same, but different proportions, the curative effect is not the same. Different composition of the pharmacodynamic components or different proportions of the pharmacodynamic components represent different traditional Chinese medicine, with different clinical efficacy, the root cause lies in the different pharmacodynamic components should be distinguished. Therefore, the study and discussion of the pharmacodynamic components of Chinese medicine is the key to resolve the compatibility mechanism of traditional Chinese medicine.