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整合蛋白α 5β 1是一类存在于细胞表面的细胞粘附分子，在肝癌的发生发展和演进中起着重要的作用。研究了快速定量检测整合蛋白α 5β 1 mRNA表达的 RT－ PCR方法，结果表明：在 PCR反应体系中加入 25 ng到 200 ng mRNA的逆转录产物呈较好的线性关系，并以此为反应条件测定 pcDNA3－α 5， pcDNA3－β 1质粒共转染或单转染的α 5β 1.6－ 7721, α 5.3－ 7721, β 1.6－ 7721细胞株 mRNA的表达，表明经 pcDNA3－α 5， pcDNA3－α 1质粒感染后人肝癌细胞株 SMMC7721细胞中整合蛋白α 5β 1的表达有较大幅度的提高，而且α5，β1亚基间可能存在相互的诱导作用。
Integrin α 5β 1 is a type of cell adhesion molecule present on the cell surface and plays an important role in the development and progression of hepatocellular carcinoma. The RT-PCR method for rapid and quantitative detection of integrin α 5β 1 mRNA expression was studied. The results showed that the reverse transcription products with 25 ng to 200 ng of mRNA in the PCR reaction system showed a good linear relationship, and as a reaction condition The expression of pcDNA3-α5, pcDNA3-β1 plasmid co-transfection or single transfected α5β1.6-7721, α5.3-7721, β1.6-7721 cell line mRNA expression, indicating that by pcDNA3-α5, pcDNA3-α 1 plasmid, the expression of integrin α 5β 1 in human hepatocellular carcinoma cell line SMMC7721 increased significantly, and the interaction between α5 and β1 subunits might be induced.