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测定并分析了长江水系汨罗江4尾鳤鱼(Ochetobius elongates)和珠江水系柳江2尾鳤鱼的线粒体细胞色素b基因部分序列981bp,发现6个单倍型,其中19个变异位点,简约信息位点15个;AT含量和GC含量分别为57.6%和42.4%。在基于Kimura双参数模型的邻接树上,汩罗江和柳江鳤鱼各自聚群。汨罗江4尾鳤鱼间的遗传距离为0.001～0.007,柳江2尾鳤鱼间遗传距离为0.001,而2群体间的平均遗传距离为0.015(0.013～0.017),群体间的遗传变异大于群体内的遗传变异,表明汩罗江鳤鱼与柳江鳤鱼出现较明显的遗传分化。由于本研究采集地理群体少,分析的样本小,为更好地揭示长江和珠江水系鳤鱼的遗传变异,上述结论尚需进一步分析更多大样本的地理群体加以验证。
A total of 981bp fragments of mitochondrial cytochrome b gene were detected and analyzed in Ochetobius elongates from the Miluojiang River in the Yangtze River and Changjiang River in the Pearl River water system. There were 6 haplotypes, 19 of which were found to be polymorphic 15 loci; AT content and GC content were 57.6% and 42.4% respectively. On the adjacent trees based on the Kimura two-parameter model, the Milu River and Liujiang anchovy cluster respectively. The genetic distance between 4 catfish in Miluojiang River ranged from 0.001 to 0.007, and the genetic distance between 2 catfish from Liujiang River was 0.001, while the average genetic distance between 2 groups was 0.015 (0.013 ~ 0.017). The genetic variation among populations was larger than that in the population The genetic variation of Millettia macclurei showed that there was obvious genetic differentiation between Millettia macrophylla and Liujiang anchovy. Due to the small number of collected geographies and the small sample size in this study, the above conclusions need to be further analyzed to verify the geographical distribution of more large samples in order to better reveal the genetic variation of anchovy in the Yangtze River and Pearl River.