论文部分内容阅读
采用垂直板不连续系统聚丙烯酰胺凝胶电泳的方法，对唐菖蒲同一品种的花药、花瓣、叶片、籽球及不同品种花药的过氧化物酶同工酶谱进行了研究。结果表明：唐菖蒲过氧化物酶同工酶谱在不同器官间存在着显著差异。花药的酶谱丰富，供试７个品种的花药过氧化物酶同工酶谱共显现１１条酶带，品种间的酶带变异在４～８条之间，依据酶带的泳动速率（Ｒｆ值），可明显分为快慢两个带区，快带区Ｒｆ０．６４～０．７８，有５条酶带，慢带区Ｒｆ０．０８～０．２８，有６条酶带。叶片、籽球和花瓣的酶谱Ｒｆ仅显现出慢带区。四种器官中，花瓣和籽球的同工酶谱活性较弱，花药和叶片的同工酶谱活性较强，且酶带清晰。据此认为唐菖蒲过氧化物酶同工酶谱研究应以花药为最理想试材。
The peroxidase isozymes of anther, petal, leaf, seed bulb and anthers of the same species of gladiolus were studied by vertical plate polyacrylamide gel electrophoresis. The results showed that there was a significant difference in peroxidase isozymes between different organs of gladiolus. There were 11 bands of anther peroxidase isozymes in 7 varieties tested, and the variation of the bands among varieties was between 4 and 8, Rf value), can be divided into two fast and slow zone, fast zone Rf0.64 ~ 0.78, there are five enzyme bands, slow zone Rf0.08 ~ 0.28, there are 6 enzyme bands. The zymogram Rf of leaves, seeds and petals only showed a slow band. In the four organs, isozyme activity of petal and seed ball was weaker, isoenzyme activity of anther and leaf were stronger, and the enzyme band was clear. Therefore, the study on peroxidase isozymes in gladiolus should be based on anther as the best test material.