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应用８ Ｂｒｃ Ａ Ｍ Ｐ 和６０ Ｃｏ 照射体外诱导 Ｈｅｌａ 细胞凋亡。探讨ｂｘ Ｌ２ ，ｗ ｐ５３ ，ｍ ｐ５３ ，ｃｍ ｙｃｉ Ｎｏｓ 的基因表达和ｉ Ｎｏｓ ， Ｆａｓ 蛋白表达之间的关系，为探讨无毒性８ Ｂｒｃ Ａ Ｍ Ｐ 对宫颈癌治疗的新途径提供依据。采用 Ｔ Ｕ Ｎ Ｅ Ｌ 法和 Ｆｅｕｌｇｅｎ 法进行细胞凋亡实验；采用本实验室创新的完整细胞原位 Ｒ Ｎ Ａ 斑点印迹技术检测 Ｆａｓ 和ｉ Ｎｏｓ蛋白，应用高速薄层色谱扫描仪对斑点印迹信号进行定量测定。结果８ Ｂｒｃ Ａ Ｍ Ｐ 和６０ Ｃｏ 照射均可上调 Ｈｅｌａ ｗｐ５３ ，ｉ Ｎｏｓ 的基因表达；下调ｍ ｐ５３ ，ｂｃｌ２ ，ｃｍｙｃ 的基因表达。可增强ｉ Ｎｏｓ 蛋白质的酶活性，降低 Ｆａｓ 蛋白质的免疫反应性。显示出８ Ｂｒｃ Ａ Ｍ Ｐ 可能代替放疗作为一种治疗宫颈癌的新途径。
The apoptosis of Hela cells was induced by 8 Brc A M P and 60 Co irradiation in vitro. To investigate the relationship between gene expression of bx L2, w p53, m p53, cm yci Nos and i Nos, Fas protein expression, in order to investigate the nontoxic 8 Brc A M P treatment of cervical cancer Ways to provide a basis. TU N E L method and Feulgen method were used to carry out apoptosis test. Fas and iNOS proteins were detected by in-situ R N A dot blot assay in our laboratory. High-speed TLC scanner was used to detect dot blot signal Quantitative determination. Results 8 Br c A M P and 60 Co irradiation can increase Hela wp53, i Nos gene expression; down m p53, bcl 2, c myc gene expression. Can enhance i Nos protein enzyme activity, reduce Fas protein immunoreactivity. 8 Br c A M P may show instead of radiotherapy as a new way to treat cervical cancer.