论文部分内容阅读
目的比较手足口患儿粪便和咽拭子中肠道病毒的载量和检出率。方法收集2013年5月-2013年9月杭州市儿童医院临床诊断手足口病患儿1 117例标本(其中粪便105例,咽拭子1 012例),采用荧光定量RT-PCR方法进行肠道病毒核酸检测,并对其中72例同时留取粪便和咽拭子的患儿样本分别进行阳性率和定量结果 log值配对分析。结果粪便和咽拭子标本肠道病毒总阳性率分别为86.7%、77.3%,差异有统计学意义(χ2=4.92,P=0.027);72例配对标本结果同为阳性的54例,同为阴性的11例,粪便阳性、咽拭子阴性为7例,粪便阴性、咽拭子阳性为0例,差异有统计学意义(Mc Nemar P=0.016);72例配对标本定量结果显示粪便标本中病毒含量高于咽拭子标本(t=6.924,P=0.000)。结论粪便标本中肠道病毒检出率高于咽拭子标本,且病毒载量也高于咽拭子标本。
Objective To compare the viral load and detection rate of enterovirus in feces and throat swabs of hand, foot and mouth children. Methods A total of 1 117 clinical samples of hand-foot-mouth disease diagnosed in Hangzhou Children’s Hospital from May 2013 to September 2013 were collected, including 105 cases of stool and 1 012 cases of pharyngeal swab. Fluorescent quantitative RT-PCR was performed for intestinal Virus nucleic acid test, and 72 cases of both samples of feces and throat swab samples were positive rate and quantitative results of log-value paired analysis. Results The total positive rates of enterovirus in feces and throat swab specimens were 86.7% and 77.3%, respectively, with significant difference (χ2 = 4.92, P = 0.027). The results of 72 paired specimens were the same as those of positive ones Negative in 11 cases, positive stool, throat swab was negative in 7 cases, stool negative, throat swab was positive in 0 cases, the difference was statistically significant (Mc Nemar P = 0.016); 72 pairs of specimens quantitative results showed that stool specimens The virus content was higher than throat swab specimens (t = 6.924, P = 0.000). Conclusion The detection rate of enterovirus in stool specimens is higher than that of throat swab specimens, and the viral load is also higher than that of throat swab specimens.